Profiling of antioxidative proteolysate enzymatically hydrolysed from stone fish (Actinopyga lecanora)

Ebrahimpour A., . and Chay S. Y., . and M. Zarei, . and N. Saari, . and Bordbar S., . Profiling of antioxidative proteolysate enzymatically hydrolysed from stone fish (Actinopyga lecanora). pp. 848-859. ISSN 22317546

Marine livings represent one of the richest sources of protein with valuable bioactives. The present work explores the antioxidative potential of stone fish a sea cucumber species typically discarded as by-catch. Stone fish was enzymatically hydrolysed using papain and the resulting proteolysate exhibited strong antioxidant activity in DPPH radical scavenging (IC‚‚ 0.49 mg/mL) ABTS (IC‚‚ 0.36 mg/mL) radical scavenging and FRAP value (0.29 mM FeSO‚„ ) after 8 h of hydrolysis. Fractionation of proteolysate was then performed using three approaches namely ultrafiltration reversed-phase high performance liquid chromatography and isoelectric focusing techniques to profile and characterise the antioxidative proteolysate. Results indicated that papain-generated proteolysate from stone fish flesh possessed considerable amount of antioxidative peptides with molecular weight of approximately 2 kDa low hydrophobicity ( 20) and pI 9.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/28%20(04)%202021/DONE%2...

Abstract

Marine livings represent one of the richest sources of protein with valuable bioactives. The present work explores the antioxidative potential of stone fish a sea cucumber species typically discarded as by-catch. Stone fish was enzymatically hydrolysed using papain and the resulting proteolysate exhibited strong antioxidant activity in DPPH radical scavenging (IC‚‚ 0.49 mg/mL) ABTS (IC‚‚ 0.36 mg/mL) radical scavenging and FRAP value (0.29 mM FeSO‚„ ) after 8 h of hydrolysis. Fractionation of proteolysate was then performed using three approaches namely ultrafiltration reversed-phase high performance liquid chromatography and isoelectric focusing techniques to profile and characterise the antioxidative proteolysate. Results indicated that papain-generated proteolysate from stone fish flesh possessed considerable amount of antioxidative peptides with molecular weight of approximately 2 kDa low hydrophobicity ( 20) and pI 9.

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Item Type:	Article
AGROVOC Term:	Marine resources
AGROVOC Term:	Hydrolysis
AGROVOC Term:	Sampling
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Analysis of variance
AGROVOC Term:	Nutritional value
AGROVOC Term:	Antioxidants
AGROVOC Term:	Food products
AGROVOC Term:	Papain
AGROVOC Term:	Hydrolysis
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10007

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