Evaluation of the DPPH radical scavenging activity total phenolic content and total flavanoid content of different solvent extracts of Catunaregam tomentosa (Blume ex DC) tirveng leaves

Ahmad Syibli Othman, . and Syed Ahmad Tajudin Tuan Johari, . and Abdul Manaf Ali, . and Wan Amalina Wan Mamat, . and Muhammad Yusran Abdul Aziz, . Evaluation of the DPPH radical scavenging activity total phenolic content and total flavanoid content of different solvent extracts of Catunaregam tomentosa (Blume ex DC) tirveng leaves. pp. 1-7. ISSN 2180-1983

Catunaregam tomentosa(Blume ex DC) Tirveng is commonly known as Khet in Thailand and Bisa Ular or Badang in Malaysia. The tree is widely distributed in the north-east region of Thailand while in Malaysia the tree usually grows in the open waterfront area at Terengganu. This plant belongs to the Rubiaceae family and the genus catunaregam has interesting pharmacological activities such as anti-inflammatory antispasmodic antidysenteric antifertility and immunomodulatory. In this study the leaves were extracted using dichloromethane ethyl acetate and ethanol. Total phenolic was determined by Folin-Ciocalteau method are 115.01.135 340.83.209and 30.241.702mg GAE/g with dichloromethane ethanol and ethyl acetate respectively. Total flavonoid was determined by the aluminium chloride calorimetric methodare 47.010.577 56.781.970and 40.891.079mg QE/g with dichloromethane ethanol and ethyl acetate respectively. Meanwhile its antioxidant activity was evaluated by the 2 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanolic extract was found to have the highest percentages of phenolic and flavonoid content.The ethanolic extract also demonstrated strong DPPH scavenging activity with IC50 at 20.070.51g/mL.

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Abstract

Catunaregam tomentosa(Blume ex DC) Tirveng is commonly known as Khet in Thailand and Bisa Ular or Badang in Malaysia. The tree is widely distributed in the north-east region of Thailand while in Malaysia the tree usually grows in the open waterfront area at Terengganu. This plant belongs to the Rubiaceae family and the genus catunaregam has interesting pharmacological activities such as anti-inflammatory antispasmodic antidysenteric antifertility and immunomodulatory. In this study the leaves were extracted using dichloromethane ethyl acetate and ethanol. Total phenolic was determined by Folin-Ciocalteau method are 115.01.135 340.83.209and 30.241.702mg GAE/g with dichloromethane ethanol and ethyl acetate respectively. Total flavonoid was determined by the aluminium chloride calorimetric methodare 47.010.577 56.781.970and 40.891.079mg QE/g with dichloromethane ethanol and ethyl acetate respectively. Meanwhile its antioxidant activity was evaluated by the 2 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanolic extract was found to have the highest percentages of phenolic and flavonoid content.The ethanolic extract also demonstrated strong DPPH scavenging activity with IC50 at 20.070.51g/mL.

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Item Type:	Article
AGROVOC Term:	Rubiaceae
AGROVOC Term:	Phenolic content
AGROVOC Term:	Flavonoids
AGROVOC Term:	Random sampling
AGROVOC Term:	Antioxidants
AGROVOC Term:	Medicinal plants
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10027

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