Effect of carbon sources on different strains of phytase-producing bacteria isolated from Malaysias Hot Spring

Nurul Asma Hasliza Zulkifly, . and Nurul Izyan Che Mohamood, . and Nadiawati Alias, . Effect of carbon sources on different strains of phytase-producing bacteria isolated from Malaysias Hot Spring. pp. 29-38. ISSN 2180-1983

Animal feed from cereal grains and oilseed meals mainly containing phytic acid which has adverse effects on animal nutrition and its environment. Ruminants can easily digest the phytic acid as they have fungi and bacteria in their guts which can produce phytase to degrade the phytic acid. Meanwhile phytic acid in non-ruminant animals is poorly digested due to the lack of sufficient phytase in their guts. Thus the feed must be supplemented with inorganic phosphate to ensure it can absorb adequate nutrients. This study aimed to determine the effects of using different carbon sources to the growth of different strains of phytase producing bacteria based on optical density (OD) colony forming unit (CFU) and their phytase production. All four strains of potentially producing-phytase bacteria have been isolated from several hot springs in Malaysia. The bacteria were grown in modified Phytase Screening Medium (PSM) with glucose and lactose as a carbon source and under optimum culture conditions (pH 5.5 37 C 200 rpm) for 72 hours. For quantitative screening of phytase production the bacterial cultures were harvested to obtain the supernatants that were used to measure the amount of inorganic phosphorus released by the bacterial strains. Among these carbon sources glucose has shown consistency between their CFU counts and the observed ODs whereas lactose shown inconsistency. Meanwhile the maximum phytase activity was recorded for all strains in the presence of glucose in which bacteria strain L3 (0.0404 U/mL) RT (0.0359 U/mL) B9 (0.0262 U/mL) and A (0.0263 U/mL). As for the overall strain L3 (Labis Johor) gave a promising rate of inorganic phosphate released with optimum phytase activity value of 0.0404 U/mL in presence of glucose and lactose. The optimisation of the fermentation medium can contribute to more economical production of industrial enzyme as phytase has the potential to produce feed additives for poultry feeding.

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Animal feed from cereal grains and oilseed meals mainly containing phytic acid which has adverse effects on animal nutrition and its environment. Ruminants can easily digest the phytic acid as they have fungi and bacteria in their guts which can produce phytase to degrade the phytic acid. Meanwhile phytic acid in non-ruminant animals is poorly digested due to the lack of sufficient phytase in their guts. Thus the feed must be supplemented with inorganic phosphate to ensure it can absorb adequate nutrients. This study aimed to determine the effects of using different carbon sources to the growth of different strains of phytase producing bacteria based on optical density (OD) colony forming unit (CFU) and their phytase production. All four strains of potentially producing-phytase bacteria have been isolated from several hot springs in Malaysia. The bacteria were grown in modified Phytase Screening Medium (PSM) with glucose and lactose as a carbon source and under optimum culture conditions (pH 5.5 37 C 200 rpm) for 72 hours. For quantitative screening of phytase production the bacterial cultures were harvested to obtain the supernatants that were used to measure the amount of inorganic phosphorus released by the bacterial strains. Among these carbon sources glucose has shown consistency between their CFU counts and the observed ODs whereas lactose shown inconsistency. Meanwhile the maximum phytase activity was recorded for all strains in the presence of glucose in which bacteria strain L3 (0.0404 U/mL) RT (0.0359 U/mL) B9 (0.0262 U/mL) and A (0.0263 U/mL). As for the overall strain L3 (Labis Johor) gave a promising rate of inorganic phosphate released with optimum phytase activity value of 0.0404 U/mL in presence of glucose and lactose. The optimisation of the fermentation medium can contribute to more economical production of industrial enzyme as phytase has the potential to produce feed additives for poultry feeding.

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Item Type:	Article
AGROVOC Term:	Carbon
AGROVOC Term:	Strain differences
AGROVOC Term:	Bacteria
AGROVOC Term:	Sampling
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Phytase
AGROVOC Term:	Pharmacology
AGROVOC Term:	Phytic acid
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10030

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