Comparison of sample preparation methods and chemical assays for determination of titanium phosphorus and calcium in broiler diets and digesta

Samat N., . and Miller H., . Comparison of sample preparation methods and chemical assays for determination of titanium phosphorus and calcium in broiler diets and digesta. pp. 11-19. ISSN 2550-2123

In understanding the effectiveness of phytase in improving nutrient digestibility digesta samples from each segment of the gastrointestinal tract (GIT) need to be analysed for several different nutrients which require a large amount of dry matter. This study aimed to develop a typical sample preparation that could minimize the amount of digesta samples required and enable sequential chemical analyses. The specific objectives were to compare the content of titanium (Ti) phosphorus (P) and calcium (Ca) in diet and digesta samples after being separately prepared using different acid digestion and analysed by different chemical methods. In this study gizzard and terminal ileal digesta were collected from 20 d old Ross 308 male broilers fed with wheat/corn diets either with or without supplemental phytase at 1500 FTU/kg. Diets and digesta samples were digested in hydrochloric acid (HCl) and sulphuric acid (H2SO4) and the contents of Ti P and Ca were determined by colourimetry and ICP-OES methods. The present study showed that H2SO4 could be used to replace HCl in sample digestion before mineral analysis. Furthermore digestion of diet and digesta using H2SO4 enabled sequential analysis of Ti P and other minerals.

Full text available from: Official URL: https://mjas.my/mjas-v2/rf/pages/journal/v23i2/2-c...

Abstract

In understanding the effectiveness of phytase in improving nutrient digestibility digesta samples from each segment of the gastrointestinal tract (GIT) need to be analysed for several different nutrients which require a large amount of dry matter. This study aimed to develop a typical sample preparation that could minimize the amount of digesta samples required and enable sequential chemical analyses. The specific objectives were to compare the content of titanium (Ti) phosphorus (P) and calcium (Ca) in diet and digesta samples after being separately prepared using different acid digestion and analysed by different chemical methods. In this study gizzard and terminal ileal digesta were collected from 20 d old Ross 308 male broilers fed with wheat/corn diets either with or without supplemental phytase at 1500 FTU/kg. Diets and digesta samples were digested in hydrochloric acid (HCl) and sulphuric acid (H2SO4) and the contents of Ti P and Ca were determined by colourimetry and ICP-OES methods. The present study showed that H2SO4 could be used to replace HCl in sample digestion before mineral analysis. Furthermore digestion of diet and digesta using H2SO4 enabled sequential analysis of Ti P and other minerals.

[error in script]

Item Type:	Article
AGROVOC Term:	Broilers
AGROVOC Term:	Animal feeding
AGROVOC Term:	Chemical analysis (methods)
AGROVOC Term:	Diet
AGROVOC Term:	Digesta
AGROVOC Term:	Phosphorus
AGROVOC Term:	Calcium
AGROVOC Term:	Digestibility
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10053

Actions (login required)

View Item