Optimization and characterization of biocellulose production from bacteria isolated from passion fruits

Nadiawati Alias, . and Muhammad Haikal Hizani, . and Syamil Choo Shaiful Bahri, . and Khairul Anuar Apendi, . Optimization and characterization of biocellulose production from bacteria isolated from passion fruits. pp. 21-30. ISSN 2180-1983

Biocellulose is a strong polymer consisting of nano fibrillar structures that produce a large surface area and a microporous structure. This organic polymeris greatly in demand in various industries such as the paper industry biomedical industry and cosmetics industry. In this study biocellulose production from two bacteria known as endophytic bacterium SV845 (M02) and Pantoea ananatisIADCAMBID (M03) isolated from passion fruit was selected. Three different media formulations (Media 1 Media 2 and Media 3) were used in order to optimize the biocellulose production where each media contained a different percentage of carbon sources (glucose and fructose). The highest biocellulose production (1.544 mg/mL) was demonstrated by M02 bacteria strain in Media 1 containing glucose alone which fermented at 30 C while at 37 C the highest biocellulose(BC)production was demonstrated by M03 bacteria strain at 2.078 mg/mL in media containing glucose alone (Media 1). Data on pH changes during biocellulose fermentation in all the media were set at an initial pH of 6. The final pH values were observed in the range of 5.34 to 6.08 for M02 strain and 5.95 to 8.53 for M03 strain respectively. Characterizations of biocellulose were compared to starch using Fourier Transform Infrared (FTIR) spectroscopy. FTIR analysis indicated that the absorption peaks at 3200 cm-1 and 1630 cm-1 were derived from the association of intermolecular and intramolecular hydrogen bonds and H“O“H bending vibration of the absorbed water molecules in cellulose.

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Biocellulose is a strong polymer consisting of nano fibrillar structures that produce a large surface area and a microporous structure. This organic polymeris greatly in demand in various industries such as the paper industry biomedical industry and cosmetics industry. In this study biocellulose production from two bacteria known as endophytic bacterium SV845 (M02) and Pantoea ananatisIADCAMBID (M03) isolated from passion fruit was selected. Three different media formulations (Media 1 Media 2 and Media 3) were used in order to optimize the biocellulose production where each media contained a different percentage of carbon sources (glucose and fructose). The highest biocellulose production (1.544 mg/mL) was demonstrated by M02 bacteria strain in Media 1 containing glucose alone which fermented at 30 C while at 37 C the highest biocellulose(BC)production was demonstrated by M03 bacteria strain at 2.078 mg/mL in media containing glucose alone (Media 1). Data on pH changes during biocellulose fermentation in all the media were set at an initial pH of 6. The final pH values were observed in the range of 5.34 to 6.08 for M02 strain and 5.95 to 8.53 for M03 strain respectively. Characterizations of biocellulose were compared to starch using Fourier Transform Infrared (FTIR) spectroscopy. FTIR analysis indicated that the absorption peaks at 3200 cm-1 and 1630 cm-1 were derived from the association of intermolecular and intramolecular hydrogen bonds and H“O“H bending vibration of the absorbed water molecules in cellulose.

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Item Type:	Article
AGROVOC Term:	Cellulose
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Optimization methods
AGROVOC Term:	Isolation
AGROVOC Term:	Bacteria
AGROVOC Term:	Passion fruits
AGROVOC Term:	Culture media
AGROVOC Term:	Fructose
AGROVOC Term:	Glucose
AGROVOC Term:	Adsorption
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10079

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