Effects of nitrogen sources in phytase production on bacterial strains isolated from Malaysias Hot Spring

Siti Noor Syuhada Muhammad Amin, . and Nurul Asma Hasliza Zulkifly, . and Nadiawati Alias, . and Nur Dinie Zailan, . and Afnani Alwi, . Effects of nitrogen sources in phytase production on bacterial strains isolated from Malaysias Hot Spring. pp. 31-39. ISSN 2180-1983

Efficient strategies for phytase production gained increasing importance as more applications require high amounts of phytase for the market. Four phytase-producing bacterial strains isolated from Malaysias hot springs were used in this study to determine the effect of nitrogen sources on phytase production. All of the strains were screened out by applying halozone method which shows all of the strains were definitely positive phytase producer. Phytase Screening Medium (PSM) with soybean extract as substrate was used as a cultivation medium. Optimised condition with 1.0 (w/v) of glucose (as carbon source) pH 5.5 and 37C temperature was applied. Yeast extract and peptone were used to identify optimum nitrogen source in maximum phytase production. Quantitative analysis observed were optical density colony forming unit pH values and phytase activity to identify the effect of nitrogen source in phytase production.The finding was bacterial strain L3 as the best producer in producing maximum phytase (0.2162 U/mL) with optimised condition using yeast extract as nitrogen source. Findings in this study proved that yeast extract act as the optimum nitrogen source which contribute to maximum phytase production as supported by previous studies. This study can provide an efficient strategy to produce maximum phytase as few studies stated that phytase is an application tool in functional food production that consists of myo-inositol phosphates that is believed to have important pharmacological effects.

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Efficient strategies for phytase production gained increasing importance as more applications require high amounts of phytase for the market. Four phytase-producing bacterial strains isolated from Malaysias hot springs were used in this study to determine the effect of nitrogen sources on phytase production. All of the strains were screened out by applying halozone method which shows all of the strains were definitely positive phytase producer. Phytase Screening Medium (PSM) with soybean extract as substrate was used as a cultivation medium. Optimised condition with 1.0 (w/v) of glucose (as carbon source) pH 5.5 and 37C temperature was applied. Yeast extract and peptone were used to identify optimum nitrogen source in maximum phytase production. Quantitative analysis observed were optical density colony forming unit pH values and phytase activity to identify the effect of nitrogen source in phytase production.The finding was bacterial strain L3 as the best producer in producing maximum phytase (0.2162 U/mL) with optimised condition using yeast extract as nitrogen source. Findings in this study proved that yeast extract act as the optimum nitrogen source which contribute to maximum phytase production as supported by previous studies. This study can provide an efficient strategy to produce maximum phytase as few studies stated that phytase is an application tool in functional food production that consists of myo-inositol phosphates that is believed to have important pharmacological effects.

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Item Type:	Article
AGROVOC Term:	Phytic acid
AGROVOC Term:	Phytase
AGROVOC Term:	Bacteria
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Water springs
AGROVOC Term:	Growth media
AGROVOC Term:	Yeast extracts
AGROVOC Term:	Nitrogen
AGROVOC Term:	Growth
AGROVOC Term:	Enzyme activity
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10080

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