Effect of 2 4-dichlorophenoxy acetic acid and activated charcoal on callus induction of Cocos nucifera L. hybrid MATAG in florescence

Dhiya Dalila Zawawi, . and Mohd Fahmi Abu Bakar, . and Siti Nurkhalida Abd. Kadir, . Effect of 2 4-dichlorophenoxy acetic acid and activated charcoal on callus induction of Cocos nucifera L. hybrid MATAG in florescence. pp. 51-61. ISSN 2180-1983

Cocos Nucifera Linn. Var. MATAG is a Dwarf coconut variety that had high demand in Malaysia but low supply. Vegetative propagation of high-yielding MATAG coconut by using in vitro cloning must be considered in contributing to increase coconut productivity. Thus attempts were made to develop a protocol that would enhance callogenes is as a first preliminary step towards a protocol for mass propagation of C. nucifera L. var. MATAG. The anther isolated from immature inflorescence was used as explants and cultured on modified Eeuwens Y3 media in different concentrations of 2 4-dicholorophenoxy acetic acid (2 4-D) and activated charcoal. The highest callus induction percentage (31.25 12.18) was observed in 20 mg/L 2 4-D. However 2 4-D at any level tested were not statistically significant. Callus induction media supplemented with 0.5 mg/L activated charcoal gave the highest callusing percentage (25.89 13.59 ) indicating a positive effect of activated charcoal on callusing even though the result obtained not significant compared to control (15.95 6.76 ). But activated charcoal supplemented in media produced a significant effect compared to control in reducing the percentage of browning. In conclusion media supplemented with activated charcoal produced a higher rate on callus induction and preventing tissue browning in explant. Besides that the anther and ovule explant may serve as an efficient explant to study the callus induction of C. nucifera L. var. MATAG and as a basis to screen the potential useful plant growth regulators for somatic embryogenesis.

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Cocos Nucifera Linn. Var. MATAG is a Dwarf coconut variety that had high demand in Malaysia but low supply. Vegetative propagation of high-yielding MATAG coconut by using in vitro cloning must be considered in contributing to increase coconut productivity. Thus attempts were made to develop a protocol that would enhance callogenes is as a first preliminary step towards a protocol for mass propagation of C. nucifera L. var. MATAG. The anther isolated from immature inflorescence was used as explants and cultured on modified Eeuwens Y3 media in different concentrations of 2 4-dicholorophenoxy acetic acid (2 4-D) and activated charcoal. The highest callus induction percentage (31.25 12.18) was observed in 20 mg/L 2 4-D. However 2 4-D at any level tested were not statistically significant. Callus induction media supplemented with 0.5 mg/L activated charcoal gave the highest callusing percentage (25.89 13.59 ) indicating a positive effect of activated charcoal on callusing even though the result obtained not significant compared to control (15.95 6.76 ). But activated charcoal supplemented in media produced a significant effect compared to control in reducing the percentage of browning. In conclusion media supplemented with activated charcoal produced a higher rate on callus induction and preventing tissue browning in explant. Besides that the anther and ovule explant may serve as an efficient explant to study the callus induction of C. nucifera L. var. MATAG and as a basis to screen the potential useful plant growth regulators for somatic embryogenesis.

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Item Type:	Article
AGROVOC Term:	Cocos
AGROVOC Term:	Cocos nucifera
AGROVOC Term:	Coconuts
AGROVOC Term:	Hybrids
AGROVOC Term:	Micropropagation
AGROVOC Term:	In vitro culture
AGROVOC Term:	Inflorescences
AGROVOC Term:	Plant growth regulators
AGROVOC Term:	2 4-dichlorophenoxyacetic acid
AGROVOC Term:	Activated charcoal
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10082

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