Using buffered formalin to preserve the bent-tail characteristic of goat spermatozoa for the hypo-osmotic swelling test

Nakthong Chowalit, . and Boonmasawai Sookruetai, . and Lanamtiang Yupaporn, . and Kimsakulvech Sakdichod, . and Leesombun Arpron, . and Oupala Waraluk, . Using buffered formalin to preserve the bent-tail characteristic of goat spermatozoa for the hypo-osmotic swelling test. pp. 56-63. ISSN 2550-2123

The buffered formalin concentration and adding time for the hypo-osmotic swelling (HOS) test in goat semen have never been recommended. This study examined the different buffered concentrations for fixing bent-tail spermatozoa in HOS-treated goat semen for 5-120 minutes. In experiment 1 nine HOS-treated goat semen samples were incubated at 37C for 60 minutes and bent-tail spermatozoa were evaluated as a control. At the same time the different final concentrations (0.99 4.76 9.09 and 13.04 (v/v)) of buffered formalin were added and compared with the control. In experiment 2 nine HOS-treated goat semen samples were incubated at 37C for 5 30 60 90 and 120 minutes before being evaluated for bent-tail spermatozoa. At the same time the different final concentrations (13.04 16.67 and 20 (v/v)) of buffered formalin were added to the HOS-treated semen. All buffered formalin groups samples were placed at room temperature for 24 hours before evaluation. The occurrence of bent-tail spermatozoa in the 0.99 and 4.76 (v/v) formalin groups was lower than the control and the 9.09 (v/v) trended to be lower than the control. The 13.04 (v/v) formalin group showed some periods of a decrease in bent-tail spermatozoa. The buffered formalin concentration of at least 16.67 (v/v) added to HOS-treated goat semen had the relative stability of bent-tail spermatozoa for 5-90 minutes and was no different from the non-fixative group. These results recommended the use of an optimal concentration of buffered formalin at 16.67 (v/v) to fix the bent-tail spermatozoa in HOSs-treated goat semen during 5-90 minutes of incubation. This technique will be suitable for goat semen evaluation when there is a short period of incubation.

Full text available from: Official URL: https://mjas.my/mjas-v2/rf/pages/journal/v24i1/6-2...

Abstract

The buffered formalin concentration and adding time for the hypo-osmotic swelling (HOS) test in goat semen have never been recommended. This study examined the different buffered concentrations for fixing bent-tail spermatozoa in HOS-treated goat semen for 5-120 minutes. In experiment 1 nine HOS-treated goat semen samples were incubated at 37C for 60 minutes and bent-tail spermatozoa were evaluated as a control. At the same time the different final concentrations (0.99 4.76 9.09 and 13.04 (v/v)) of buffered formalin were added and compared with the control. In experiment 2 nine HOS-treated goat semen samples were incubated at 37C for 5 30 60 90 and 120 minutes before being evaluated for bent-tail spermatozoa. At the same time the different final concentrations (13.04 16.67 and 20 (v/v)) of buffered formalin were added to the HOS-treated semen. All buffered formalin groups samples were placed at room temperature for 24 hours before evaluation. The occurrence of bent-tail spermatozoa in the 0.99 and 4.76 (v/v) formalin groups was lower than the control and the 9.09 (v/v) trended to be lower than the control. The 13.04 (v/v) formalin group showed some periods of a decrease in bent-tail spermatozoa. The buffered formalin concentration of at least 16.67 (v/v) added to HOS-treated goat semen had the relative stability of bent-tail spermatozoa for 5-90 minutes and was no different from the non-fixative group. These results recommended the use of an optimal concentration of buffered formalin at 16.67 (v/v) to fix the bent-tail spermatozoa in HOSs-treated goat semen during 5-90 minutes of incubation. This technique will be suitable for goat semen evaluation when there is a short period of incubation.

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Item Type:	Article
AGROVOC Term:	Goats
AGROVOC Term:	Animal breeding
AGROVOC Term:	Semen preservation
AGROVOC Term:	Spermatozoa
AGROVOC Term:	Formalin
AGROVOC Term:	Chemical concentration
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10084

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