Transcriptomic and proteomic studies to investigate the basal stem rot disease in oil palm seedlings

Daim, L. D. J. and Tan, B. A. and N. Ithnin, . and Ooi, T. E. K. and Normahnani M. N., . and Appleton, D. R. and Harikrishna K., . (2023) Transcriptomic and proteomic studies to investigate the basal stem rot disease in oil palm seedlings. Journal of Oil Palm Research (Malaysia), 35. pp. 340-353. ISSN 2811-4701

When oil palm is exposed to, and infected by Ganoderma boninense, the initial defence system is launched in the roots to reduce the damage caused by the disease. The present work described the transcript and protein profiles in roots of 18 months old oil palm seedlings that were exposed to the fungal pathogen for 12 months, following artificial inoculation at six months old. Three different phenotypes were observed; control (uninoculated), asymptomatic, and symptomatic (inoculated). It was found that the transcripts from the ubiquitin-mediated proteolysis pathway were common in control and asymptomatic seedlings; while proteins involved in cellular processes, and protein and sugar metabolisms were higher in abundance in asymptomatic seedlings. The transcripts involved in carbon fixation, glycolysis/gluconeogenesis, and pyruvate metabolism together with proteins responsible for stress response were identified in symptomatic seedlings. By integrating these omics data, it was observed that symptomatic seedlings were moving towards generating and storing energy for a possible defence strategy, and at the same time emitting stress signals and responses. This was in contrast with asymptomatic seedlings where regular functions such as cellular processes and carbohydrate metabolisms were found to be active.

Full text available from: Official URL: http://jopr.mpob.gov.my/wp-content/uploads/2022/09...

Abstract

When oil palm is exposed to, and infected by Ganoderma boninense, the initial defence system is launched in the roots to reduce the damage caused by the disease. The present work described the transcript and protein profiles in roots of 18 months old oil palm seedlings that were exposed to the fungal pathogen for 12 months, following artificial inoculation at six months old. Three different phenotypes were observed; control (uninoculated), asymptomatic, and symptomatic (inoculated). It was found that the transcripts from the ubiquitin-mediated proteolysis pathway were common in control and asymptomatic seedlings; while proteins involved in cellular processes, and protein and sugar metabolisms were higher in abundance in asymptomatic seedlings. The transcripts involved in carbon fixation, glycolysis/gluconeogenesis, and pyruvate metabolism together with proteins responsible for stress response were identified in symptomatic seedlings. By integrating these omics data, it was observed that symptomatic seedlings were moving towards generating and storing energy for a possible defence strategy, and at the same time emitting stress signals and responses. This was in contrast with asymptomatic seedlings where regular functions such as cellular processes and carbohydrate metabolisms were found to be active.

[error in script]

Item Type:	Article
AGROVOC Term:	oil palms
AGROVOC Term:	plant diseases
AGROVOC Term:	seedlings
AGROVOC Term:	disease resistance
AGROVOC Term:	transcriptomics
AGROVOC Term:	proteomics
AGROVOC Term:	gene expression
AGROVOC Term:	plant pathologists
AGROVOC Term:	disease management
Geographical Term:	Malaysia
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	04 Aug 2025 13:51
Last Modified:	04 Aug 2025 13:51
URI:	http://webagris.upm.edu.my/id/eprint/1010

Actions (login required)

View Item