Stability of lactoferrin and lysozyme in human milk at various temperatures and duration of storage

Pertami Sumirah Budi, . and Kasiati, . and Agus Setyo Utomo Agus Setyo, . and Siti Nur Arifah, . Stability of lactoferrin and lysozyme in human milk at various temperatures and duration of storage. pp. 271-278. ISSN 1394–035X

Introduction: Exclusive breastfeeding especially in the first six months is essential for infants as it provides nutrition and protection against various diseases. Colostrum which is found in the first breast milk produced contains various protective factors such as lactoferrin and lysozyme. Human milk can be stored at room temperature refrigerated or kept frozen. Several factors affect the stability of the bioactive content in human milk such as temperature and storage time. The aim of this study was to measure the stability of lactoferrin and lysozyme levels in human milk during the first six hours (h) at different temperatures and compare it with that of frozen human milk. Methods: Human milk samples were obtained from 11 breastfeeding mothers using certain criteria. The human milk was stored at room temperature and 4C for 1 3 and 6 h and classified as never frozen while frozen human milk was stored at -20C for 1 3 and 6 days. Measurement of the lactoferrin and lysozyme levels was performed using enzyme-linked immunosorbent assay. Results: The results showed that storage at room temperature significantly reduced lactoferrin and lysozyme levels. Lactoferrin levels in frozen human milk did not significantly decrease during the first six days. Meanwhile the lysozyme levels in frozen human milk decreased significantly. Conclusion: The levels of lactoferrin and lysozyme in frozen human milk stored for the first six days were more stable than those stored at room temperature and 4C in the first 6 h.

Full text available from: Official URL: https://www.nutriweb.org.my/mjn/publication/27-2/V...

Abstract

Introduction: Exclusive breastfeeding especially in the first six months is essential for infants as it provides nutrition and protection against various diseases. Colostrum which is found in the first breast milk produced contains various protective factors such as lactoferrin and lysozyme. Human milk can be stored at room temperature refrigerated or kept frozen. Several factors affect the stability of the bioactive content in human milk such as temperature and storage time. The aim of this study was to measure the stability of lactoferrin and lysozyme levels in human milk during the first six hours (h) at different temperatures and compare it with that of frozen human milk. Methods: Human milk samples were obtained from 11 breastfeeding mothers using certain criteria. The human milk was stored at room temperature and 4C for 1 3 and 6 h and classified as never frozen while frozen human milk was stored at -20C for 1 3 and 6 days. Measurement of the lactoferrin and lysozyme levels was performed using enzyme-linked immunosorbent assay. Results: The results showed that storage at room temperature significantly reduced lactoferrin and lysozyme levels. Lactoferrin levels in frozen human milk did not significantly decrease during the first six days. Meanwhile the lysozyme levels in frozen human milk decreased significantly. Conclusion: The levels of lactoferrin and lysozyme in frozen human milk stored for the first six days were more stable than those stored at room temperature and 4C in the first 6 h.

[error in script]

Item Type:	Article
AGROVOC Term:	Human milk
AGROVOC Term:	Breast feeding
AGROVOC Term:	Lysozyme
AGROVOC Term:	Lactoferrin
AGROVOC Term:	Temperature
AGROVOC Term:	Stability
AGROVOC Term:	Storage stability
AGROVOC Term:	Nutritional status
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10104

Actions (login required)

View Item