Diversity composition taxa biomarkers and functional genes of fish gut microbes in peat swamp forests and its converted areas in north Selangor Malaysia

Ahmad Ismail, . and Jumria Sutra, . and Syaizwan Zahmir Zulkifli, . and Saadu Hamidu, . and Amalia Mohd Hashim, . and Mohammad Noor Azmai Amal, . Diversity composition taxa biomarkers and functional genes of fish gut microbes in peat swamp forests and its converted areas in north Selangor Malaysia. pp. 617-641. ISSN 1511-3701

The aquatic organisms in peat swamp forests are under threat due to habitat degradation resulting from human activities. This study determines the fish gut microbes diversity composition taxa biomarkers and functional genes in peat swamp forests and its converted areas in North Selangor Malaysia. Three undisturbed and disturbed areas nearby the peat swamp forests were selected. First the 16S amplicon metagenomic analysis was conducted to assess the composition and diversity of bacterial communities in fish gut contents from both areas. Then Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) and Linear discriminant analysis Effect Size (LEfSe) were used to predict disease/pathogen related functional genes. This study revealed Proteobacteria Firmicutes Bacteroidetes Fusobacteria and Actinobacteria as the predominant phyla in both studied areas. In contrast bacterial community profiles of disturbed and undisturbed areas were slightly dissimilar. Metagenome predictions revealed that genes are related to metabolism environmental information processing genetic information processing cellular processes human diseases and organismal systems. Further investigation revealed six potential biomarker genes including chronic myeloid leukaemia in an undisturbed area Vibrio cholerae infection bladder cancer pathogenic Escherichia coli infection Staphylococcus aureus infection and pertussis in disturbed areas. This study revealed that the fish gut microbiome could be used as an indicator in comparing the undisturbed and disturbed ecosystems.

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Abstract

The aquatic organisms in peat swamp forests are under threat due to habitat degradation resulting from human activities. This study determines the fish gut microbes diversity composition taxa biomarkers and functional genes in peat swamp forests and its converted areas in North Selangor Malaysia. Three undisturbed and disturbed areas nearby the peat swamp forests were selected. First the 16S amplicon metagenomic analysis was conducted to assess the composition and diversity of bacterial communities in fish gut contents from both areas. Then Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) and Linear discriminant analysis Effect Size (LEfSe) were used to predict disease/pathogen related functional genes. This study revealed Proteobacteria Firmicutes Bacteroidetes Fusobacteria and Actinobacteria as the predominant phyla in both studied areas. In contrast bacterial community profiles of disturbed and undisturbed areas were slightly dissimilar. Metagenome predictions revealed that genes are related to metabolism environmental information processing genetic information processing cellular processes human diseases and organismal systems. Further investigation revealed six potential biomarker genes including chronic myeloid leukaemia in an undisturbed area Vibrio cholerae infection bladder cancer pathogenic Escherichia coli infection Staphylococcus aureus infection and pertussis in disturbed areas. This study revealed that the fish gut microbiome could be used as an indicator in comparing the undisturbed and disturbed ecosystems.

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Item Type:	Article
AGROVOC Term:	Microbial ecology
AGROVOC Term:	Microbiota
AGROVOC Term:	Biodiversity
AGROVOC Term:	Peatlands
AGROVOC Term:	Sampling
AGROVOC Term:	analysis
AGROVOC Term:	Ecosystems
AGROVOC Term:	Environmental biology
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10142

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