Citation
Siti Nor Azizah Mahamud, . and Sheau Wei Tan, . and So Youn Youn, . and Hyun Jeong Lee, . and Ji Youn Lee, . and Young Kuk Kwon, . and Aini Ideris, . and Abdul Rahman Omar, . Isolation and characterization of Newcastle disease virus subgenotype VII.2/VIIi from commercial chicken and swan in Malaysia. pp. 879-898. ISSN 1511-3701
Abstract
Frequent Newcastle disease (ND) outbreaks in poultry have been reported in Southeast Asia including Malaysia. However limited studies have been carried out on detecting the Newcastle disease virus (NDV) from non-poultry birds. In this study the detections of NDV were carried out using tissues samples from suspected ND cases from commercial chickens and swab samples of non-poultry birds captured in bird sanctuaries. Five samples from commercial chickens and one sample from black swans were found positive for ND. They were classified as velogenic NDV based on the partial sequencing of the fusion (F) gene which revealed the amino acid motif on the F cleavage site of RRQKRF�·. In addition phylogenetic analysis based on partial F gene showed that all NVD isolates are classified as class II genotype VII subgenotype VII.2 (VIIi) and are clustered together with NDVs isolated from chickens in 2017 in Indonesia. This finding indicates the occurrence of subgenotype VII.2 (VIIi) as the fifth panzootic of ND in Malaysia and the importance of the epidemiology of virulent NDV in various avian species.
Download File
Full text available from:
Official URL: http://www.pertanika.upm.edu.my/resources/files/Pe...
|
Abstract
Frequent Newcastle disease (ND) outbreaks in poultry have been reported in Southeast Asia including Malaysia. However limited studies have been carried out on detecting the Newcastle disease virus (NDV) from non-poultry birds. In this study the detections of NDV were carried out using tissues samples from suspected ND cases from commercial chickens and swab samples of non-poultry birds captured in bird sanctuaries. Five samples from commercial chickens and one sample from black swans were found positive for ND. They were classified as velogenic NDV based on the partial sequencing of the fusion (F) gene which revealed the amino acid motif on the F cleavage site of RRQKRF�·. In addition phylogenetic analysis based on partial F gene showed that all NVD isolates are classified as class II genotype VII subgenotype VII.2 (VIIi) and are clustered together with NDVs isolated from chickens in 2017 in Indonesia. This finding indicates the occurrence of subgenotype VII.2 (VIIi) as the fifth panzootic of ND in Malaysia and the importance of the epidemiology of virulent NDV in various avian species.
Additional Metadata
Item Type: | Article |
---|---|
AGROVOC Term: | Newcastle disease virus |
AGROVOC Term: | Chickens |
AGROVOC Term: | Swans |
AGROVOC Term: | Poultry |
AGROVOC Term: | Sampling |
AGROVOC Term: | Isolation techniques |
AGROVOC Term: | Gel electrophoresis |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:55 |
URI: | http://webagris.upm.edu.my/id/eprint/10157 |
Actions (login required)
![]() |
View Item |