Antioxidants properties of Murraya koenigii: a comparative study of three different extraction methods

Smedley K. L., . and Mohd Zin Z., . and Parithy M. T., . and Hasmadi M., . and Rusli N. D., . and Zainol M. K., . Antioxidants properties of Murraya koenigii: a comparative study of three different extraction methods. pp. 43-49. ISSN 2550-2166

Nutraceutical and pharmaceutical industries have been increasingly engaged in finding natural alternatives compounds as potential antioxidants. The use of phytochemicals is introduced as a good source of natural antioxidants. Murayya koenigii leaves commonly used in cooking and traditional medicines have been examined for their remarkable antioxidant potential yet still it remains an understudied herb. Therefore this study aimed to determine the antioxidant properties and flavonoids profile in M. koenigii leaves extracted using; solvent assisted extraction (SAE) microwave assisted extraction (MAE) and ultrasonic assisted extraction (UAE). The antioxidant properties of M. koenigii were analysed qualitatively and quantitatively using high performance liquid chromatography (HPLC). M. koenigii leaves extracted using the UAE method have responded strongly towards a 2 2-diphenyl -2-picryl-hydrazyl DPPH assay with the highest inhibition () of 78.001.00. Using the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) method assays the M. koenigii leaves with the lowest absorbance were assigned as a sample with the highest antioxidant activity. The M. koenigii leaves extracted using UAE had the lowest absorbance with 0.010.00. In the TPC assay the MAE method showed the highest total phenolic content (120.6014.81 mg GAE/g sample). The TFC assay demonstrated that MAE methods have the highest total phenolic content (93.384.33 mg QE/g sample. The M. koenigii leaves extracted by MAE showed the highest gallic acid catechin epigallocatechin gallate rutin and kaempferol concentration (mg/L). M. koenigii leaves subjected to SAE extraction has the highest concentration of p-coumaric acid myricetin and quercetin (mg/L). This study found that M. koenigii leaves extracted using UAE exhibited better antioxidant activities than that of MAE and SAE. These useful findings have managed to narrow the knowledge gap regarding the effects of different extraction methods on the antioxidant property of M. koenigii.

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Abstract

Nutraceutical and pharmaceutical industries have been increasingly engaged in finding natural alternatives compounds as potential antioxidants. The use of phytochemicals is introduced as a good source of natural antioxidants. Murayya koenigii leaves commonly used in cooking and traditional medicines have been examined for their remarkable antioxidant potential yet still it remains an understudied herb. Therefore this study aimed to determine the antioxidant properties and flavonoids profile in M. koenigii leaves extracted using; solvent assisted extraction (SAE) microwave assisted extraction (MAE) and ultrasonic assisted extraction (UAE). The antioxidant properties of M. koenigii were analysed qualitatively and quantitatively using high performance liquid chromatography (HPLC). M. koenigii leaves extracted using the UAE method have responded strongly towards a 2 2-diphenyl -2-picryl-hydrazyl DPPH assay with the highest inhibition () of 78.001.00. Using the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) method assays the M. koenigii leaves with the lowest absorbance were assigned as a sample with the highest antioxidant activity. The M. koenigii leaves extracted using UAE had the lowest absorbance with 0.010.00. In the TPC assay the MAE method showed the highest total phenolic content (120.6014.81 mg GAE/g sample). The TFC assay demonstrated that MAE methods have the highest total phenolic content (93.384.33 mg QE/g sample. The M. koenigii leaves extracted by MAE showed the highest gallic acid catechin epigallocatechin gallate rutin and kaempferol concentration (mg/L). M. koenigii leaves subjected to SAE extraction has the highest concentration of p-coumaric acid myricetin and quercetin (mg/L). This study found that M. koenigii leaves extracted using UAE exhibited better antioxidant activities than that of MAE and SAE. These useful findings have managed to narrow the knowledge gap regarding the effects of different extraction methods on the antioxidant property of M. koenigii.

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Item Type:	Article
AGROVOC Term:	Pharmaceutical industry
AGROVOC Term:	Traditional medicines
AGROVOC Term:	Medicinal herbs
AGROVOC Term:	Sampling
AGROVOC Term:	analysis
AGROVOC Term:	Antioxidants
AGROVOC Term:	Solvent extraction
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10162

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