Investigation of the anti-cancer and apoptotic properties of aqueous extract from fermented African locust bean seeds

Meyer M., . and Sibuyi N. R., . and Osoniyi O., . and Ayo-Lawal R. A., . and Ekpo O., . Investigation of the anti-cancer and apoptotic properties of aqueous extract from fermented African locust bean seeds. pp. 203-210. ISSN 2550-2166

Some fermented foods are reported to possess anti-cancer properties. Fermented African locust bean seeds is a condiment prepared from fermentation of Parkia biglobosa. It has been reportedly functional for various medicinal activities but not anti-cancer. The cytotoxic and apoptosis-inducing properties of the aqueous extract of the condiment were investigated in human cancer - hepatocellular (Hep-G2) and cervical (HeLa) and noncancer cell lines. Cytotoxicity was evaluated using the 3-(4 5-dimethylthiazol-2-yl)-2 5- diphenyl tetrazolium bromide (MTT) and clonogenic cell survival assays. Apoptotic cell death and DNA fragmentation were also investigated. The results revealed cytotoxicity to both cell lines in a dose-dependent manner (P 0.05) and selective activities between cancer and non-cancer cells. The IC‚‚ values were 1.3 and 0.5 mg/mL for Hep-G2 and HeLa cells respectively. Furthermore the extract induced apoptotic cell death in only Hep -G2 (73.030.73) cells. The morphologic photomicrographs correlated well with other findings indicating the cell-specific cytotoxicity of the condiment.

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Abstract

Some fermented foods are reported to possess anti-cancer properties. Fermented African locust bean seeds is a condiment prepared from fermentation of Parkia biglobosa. It has been reportedly functional for various medicinal activities but not anti-cancer. The cytotoxic and apoptosis-inducing properties of the aqueous extract of the condiment were investigated in human cancer - hepatocellular (Hep-G2) and cervical (HeLa) and noncancer cell lines. Cytotoxicity was evaluated using the 3-(4 5-dimethylthiazol-2-yl)-2 5- diphenyl tetrazolium bromide (MTT) and clonogenic cell survival assays. Apoptotic cell death and DNA fragmentation were also investigated. The results revealed cytotoxicity to both cell lines in a dose-dependent manner (P 0.05) and selective activities between cancer and non-cancer cells. The IC‚‚ values were 1.3 and 0.5 mg/mL for Hep-G2 and HeLa cells respectively. Furthermore the extract induced apoptotic cell death in only Hep -G2 (73.030.73) cells. The morphologic photomicrographs correlated well with other findings indicating the cell-specific cytotoxicity of the condiment.

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Item Type:	Article
AGROVOC Term:	Locust beans
AGROVOC Term:	Seed
AGROVOC Term:	Fermented foods
AGROVOC Term:	Parkia biglobosa
AGROVOC Term:	Cancer (disease)
AGROVOC Term:	Cytotoxicity
AGROVOC Term:	Experiments
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Apoptosis
AGROVOC Term:	Condiments
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10180

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