Calcium fixation on fortified rice made with various rice varieties

Wariyah Ch., . and Anwar C., . and Astuti M., . and Supriyadi, . Calcium fixation on fortified rice made with various rice varieties. pp. 285-290. ISSN 2550-2166

The aim of this study was to evaluate calcium fixation and calcium binding in calciumfortified rice. Calcium-fortified rice was made by soaking (infusing) rice in a Ca-lactate or Ca-gluconate solution at temperature of 80oC for 20 mins. The rice types used in this study were low- medium- and high amylose rice represented by Memberamo Ciherang and IR-42 rice varieties. Calcium retention in rice was tested by washing and dialysis and calcium levels in the rice were determined by Atomic Absorption Spectroscopy. Calcium fixation was determined by using an FT-IR infrared spectrometer based on the changes in infrared spectra of the functional groups of -OH and C-O. The research showed that calcium retention in rice after washing was between 86.23 - 94.38 (Ca-lactate) and 89.37 - 90.15 (Ca-gluconate) Ca retention after dialysis was between 37.49 to 44.13 (Ca-lactate) and 37.40 to 42.86 (Ca-gluconate). The addition of Ca-lactate or Ca-gluconate to rice caused a decrease in the absorbance value and the absorption band area square at a wave numbers 3425 cm� (-OH group) and 1300-1000 cm� (C-O stretching vibration). Based on these data and the retention of Ca� after washing and dialysis the Ca� in fortified-rice was bound by hydrogen bonding to form Ca-hydrate and by ionicdipole bonding with “OH group of starch molecules and/or trapping in gelatinized starch.

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Abstract

The aim of this study was to evaluate calcium fixation and calcium binding in calciumfortified rice. Calcium-fortified rice was made by soaking (infusing) rice in a Ca-lactate or Ca-gluconate solution at temperature of 80oC for 20 mins. The rice types used in this study were low- medium- and high amylose rice represented by Memberamo Ciherang and IR-42 rice varieties. Calcium retention in rice was tested by washing and dialysis and calcium levels in the rice were determined by Atomic Absorption Spectroscopy. Calcium fixation was determined by using an FT-IR infrared spectrometer based on the changes in infrared spectra of the functional groups of -OH and C-O. The research showed that calcium retention in rice after washing was between 86.23 - 94.38 (Ca-lactate) and 89.37 - 90.15 (Ca-gluconate) Ca retention after dialysis was between 37.49 to 44.13 (Ca-lactate) and 37.40 to 42.86 (Ca-gluconate). The addition of Ca-lactate or Ca-gluconate to rice caused a decrease in the absorbance value and the absorption band area square at a wave numbers 3425 cm� (-OH group) and 1300-1000 cm� (C-O stretching vibration). Based on these data and the retention of Ca� after washing and dialysis the Ca� in fortified-rice was bound by hydrogen bonding to form Ca-hydrate and by ionicdipole bonding with “OH group of starch molecules and/or trapping in gelatinized starch.

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Item Type:	Article
AGROVOC Term:	Calcium binding proteins
AGROVOC Term:	Rice
AGROVOC Term:	Nutrient uptake
AGROVOC Term:	Crop diversification
AGROVOC Term:	Plant breeding
AGROVOC Term:	Food fortification
AGROVOC Term:	Amylose
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10190

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