Antioxidant activities and simultaneous HPLC-DAD profiling of polyphenolic compounds from Moringa oleifera Lam. leaves grown in Bangladesh


Citation

Ahmed K. S., . and Jahan I. A., . and Jahan F., . and Hossain H., . Antioxidant activities and simultaneous HPLC-DAD profiling of polyphenolic compounds from Moringa oleifera Lam. leaves grown in Bangladesh. pp. 401-408. ISSN 2550-2166

Abstract

Moringa oleifera Lam. has been used as a traditional medicine for the treatment of several diseases. In this study the antioxidant activity and simultaneous HPLC profiling of polyphenolic compounds in 80 ethanol and water extracts of M. oleifera tender and mature leaves available in Bangladesh were investigated. The 80 ethanol extract of M. oleifera tender leaves contained high concentration and more amount of 3 4- dihydroxybenzoic acid (-) epicatechin and rosmarinic acid (201.321.94 213.081.96 and 133.841.42 mg/100 g of dry extract respectively) than that of mature leaves (82.551.09 141.861.10 and 16.230.76 mg/100 g of dry extract respectively). On the other hand the water extract of M. oleifera tender leaves contained a high concentration of catechin hydrate trans-ferulic acid and quercetin (530.051.91 166.380.90 and 771.741.34 mg/100 g of dry extract respectively) than that of mature leaves (207.210.90 62.420.45 and 483.251.04 mg/100 g of dry extract respectively). It was also found that 3 4-dihydroxybenzoic acid catechol p-coumaric acid and trans-cinnamic acid were detected only in 80 ethanol extract of M. oleifera leaves but syringic acid was identified in the water extract. In DPPH free radical scavenging activity test IC‚‚ values of 80 ethanol extract of tender leaves (EETL) 80 ethanol extract of mature leaves (EEML) water extract of tender leaves (WETL) and water extract of mature leaves (WEML) were found to be moderately significant (105.501.05 115.001.14 216.001.34 and 269.501.56 g/mL respectively) when compared to the IC‚‚ value of ascorbic acid 3.320.07 g/mL. The polyphenolic compounds are responsible for antioxidant activity. On the basis of the present study it can be suggested that the leaves show antioxidant activity and its contained a significant amount and different types of polyphenolic compounds. Standardized polyphenolic compounds from M. oleifera leaves could be used as a better source of natural antioxidant supplement.


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Abstract

Moringa oleifera Lam. has been used as a traditional medicine for the treatment of several diseases. In this study the antioxidant activity and simultaneous HPLC profiling of polyphenolic compounds in 80 ethanol and water extracts of M. oleifera tender and mature leaves available in Bangladesh were investigated. The 80 ethanol extract of M. oleifera tender leaves contained high concentration and more amount of 3 4- dihydroxybenzoic acid (-) epicatechin and rosmarinic acid (201.321.94 213.081.96 and 133.841.42 mg/100 g of dry extract respectively) than that of mature leaves (82.551.09 141.861.10 and 16.230.76 mg/100 g of dry extract respectively). On the other hand the water extract of M. oleifera tender leaves contained a high concentration of catechin hydrate trans-ferulic acid and quercetin (530.051.91 166.380.90 and 771.741.34 mg/100 g of dry extract respectively) than that of mature leaves (207.210.90 62.420.45 and 483.251.04 mg/100 g of dry extract respectively). It was also found that 3 4-dihydroxybenzoic acid catechol p-coumaric acid and trans-cinnamic acid were detected only in 80 ethanol extract of M. oleifera leaves but syringic acid was identified in the water extract. In DPPH free radical scavenging activity test IC‚‚ values of 80 ethanol extract of tender leaves (EETL) 80 ethanol extract of mature leaves (EEML) water extract of tender leaves (WETL) and water extract of mature leaves (WEML) were found to be moderately significant (105.501.05 115.001.14 216.001.34 and 269.501.56 g/mL respectively) when compared to the IC‚‚ value of ascorbic acid 3.320.07 g/mL. The polyphenolic compounds are responsible for antioxidant activity. On the basis of the present study it can be suggested that the leaves show antioxidant activity and its contained a significant amount and different types of polyphenolic compounds. Standardized polyphenolic compounds from M. oleifera leaves could be used as a better source of natural antioxidant supplement.

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Moringa oleifera
AGROVOC Term: Leaf extracts
AGROVOC Term: HPLC
AGROVOC Term: Ethanol
AGROVOC Term: Water
AGROVOC Term: Polyphenol content
AGROVOC Term: Antioxidants
AGROVOC Term: Natural antioxidants
AGROVOC Term: Production potential
AGROVOC Term: Food industry
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:55
URI: http://webagris.upm.edu.my/id/eprint/10233

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