Molecular cloning of Elaeis guineensis phytoene synthase (Egpsy) and its expression in mesocarp tissues

Wan Nur Syuhada W. S., . and Rasid O. A., . and Ghulam Kadir Ahmad Parveez, . Molecular cloning of Elaeis guineensis phytoene synthase (Egpsy) and its expression in mesocarp tissues. pp. 447-457. ISSN 2811-4701

Phytoene synthase (psy) gene is responsible for the synthesis of carotenoids in plants. In this study complimentary deoxyribonucleic acid (cDNA) and genomic sequences of psy were isolated by rapid amplification of cDNA ends and polymerase chain reaction (PCR) using ribonucleic acid (RNA) and DNA isolated from the mesocarp tissues of oil palm (Elaeis guineensis). The size of the full-length cDNA was 2233 bp. The open reading frame (ORF) was 1299 bp and encoded 433 deduced amino acid (AA) residues. The transcript profile revealed that psy was expressed at a maximum level at 12 weeks after anthesis (WAA). The lowest expression of psy was at 13 WAA which was speculated as the transition of the leaf-like carotenoid to the formation of storage carotenoids. The AA sequence deduced from the cDNA was 70- 78 identical to PSY from other higher plants. The genomic sequence analysis revealed that the psy gene contains six exons and five introns. Southern blot analysis indicated that oil palm has only one copy of the psy gene in its genome.

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Abstract

Phytoene synthase (psy) gene is responsible for the synthesis of carotenoids in plants. In this study complimentary deoxyribonucleic acid (cDNA) and genomic sequences of psy were isolated by rapid amplification of cDNA ends and polymerase chain reaction (PCR) using ribonucleic acid (RNA) and DNA isolated from the mesocarp tissues of oil palm (Elaeis guineensis). The size of the full-length cDNA was 2233 bp. The open reading frame (ORF) was 1299 bp and encoded 433 deduced amino acid (AA) residues. The transcript profile revealed that psy was expressed at a maximum level at 12 weeks after anthesis (WAA). The lowest expression of psy was at 13 WAA which was speculated as the transition of the leaf-like carotenoid to the formation of storage carotenoids. The AA sequence deduced from the cDNA was 70- 78 identical to PSY from other higher plants. The genomic sequence analysis revealed that the psy gene contains six exons and five introns. Southern blot analysis indicated that oil palm has only one copy of the psy gene in its genome.

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Item Type:	Article
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palm
AGROVOC Term:	Mesocarp
AGROVOC Term:	Molecular cloning
AGROVOC Term:	Gene expression
AGROVOC Term:	Plant physiology
AGROVOC Term:	Sampling
AGROVOC Term:	analysis
AGROVOC Term:	Production data
AGROVOC Term:	Carotenoids
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10252

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