Pectinase production from banana peel biomass via the optimization of the solid-state fermentation conditions of Aspergillus niger strain

Nazaitulshila Rasit, . and Yong Sin Sze, . and Mohd Ali Hassan, . and Ooi Chee Kuan, . and Sofiah Hamzah, . and Wan Rafizah Wan Abdullah@Wan Abd. Rahman, . and Md. Nurul Islam Siddique, . Pectinase production from banana peel biomass via the optimization of the solid-state fermentation conditions of Aspergillus niger strain. pp. 257-275. ISSN 2231-8526

In this study the biomass of banana peel was used to produce pectinase via optimization of solid-state fermentation conditions of the filamentous fungi Aspergillus nigeA. niger). The operating conditions of solid-state fermentation were optimized using the method of full factorial design with incubation temperature ranging between 25 C and 35 C moisture content between 40 and 60 and inoculum size between 1.6 x 106 spores/mL and 1.4 x 107 spores/mL. Optimizing the solid-state fermentation conditions appeared crucial to minimize the sample used in this experimental design and determine the significant correlation between the operating conditions. A relatively high maximal pectinase production of 27 UmL-1 was attained at 35 C of incubation 60 of moisture content and 1.6 x 106 spores/mL of inoculum size with a relatively low amount of substrate (5 g). Given that the production of pectinase with other substrates (e.g. pineapple waste lemon peel cassava waste and wheat bran) generally ranges between 3 U/mL and 16 U/mL (Abdullah et al. 2018; Handa et al. 2016; Melnichuk et al. 2020; Thangaratham and Manimegalai 2014; Salim et al. 2017) thus the yield of pectinase derived from the banana peel in this study (27 U/mL) was considered moderately high. The findings of this study indicated that the biomass of banana peel would be a potential substrate for pectinase production via the solid-state fermentation of A. niger.

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Abstract

In this study the biomass of banana peel was used to produce pectinase via optimization of solid-state fermentation conditions of the filamentous fungi Aspergillus nigeA. niger). The operating conditions of solid-state fermentation were optimized using the method of full factorial design with incubation temperature ranging between 25 C and 35 C moisture content between 40 and 60 and inoculum size between 1.6 x 106 spores/mL and 1.4 x 107 spores/mL. Optimizing the solid-state fermentation conditions appeared crucial to minimize the sample used in this experimental design and determine the significant correlation between the operating conditions. A relatively high maximal pectinase production of 27 UmL-1 was attained at 35 C of incubation 60 of moisture content and 1.6 x 106 spores/mL of inoculum size with a relatively low amount of substrate (5 g). Given that the production of pectinase with other substrates (e.g. pineapple waste lemon peel cassava waste and wheat bran) generally ranges between 3 U/mL and 16 U/mL (Abdullah et al. 2018; Handa et al. 2016; Melnichuk et al. 2020; Thangaratham and Manimegalai 2014; Salim et al. 2017) thus the yield of pectinase derived from the banana peel in this study (27 U/mL) was considered moderately high. The findings of this study indicated that the biomass of banana peel would be a potential substrate for pectinase production via the solid-state fermentation of A. niger.

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Item Type:	Article
AGROVOC Term:	Bananas
AGROVOC Term:	Peel
AGROVOC Term:	Biomass
AGROVOC Term:	Aspergillus niger
AGROVOC Term:	Optimization methods
AGROVOC Term:	Solid state fermentation
AGROVOC Term:	Pectinase
AGROVOC Term:	Agricultural waste management
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10276

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