Citation
Nazaitulshila Rasit, . and Yong Sin Sze, . and Mohd Ali Hassan, . and Ooi Chee Kuan, . and Sofiah Hamzah, . and Wan Rafizah Wan Abdullah@Wan Abd. Rahman, . and Md. Nurul Islam Siddique, . Pectinase production from banana peel biomass via the optimization of the solid-state fermentation conditions of Aspergillus niger strain. pp. 257-275. ISSN 2231-8526
Abstract
In this study the biomass of banana peel was used to produce pectinase via optimization of solid-state fermentation conditions of the filamentous fungi Aspergillus nigeA. niger). The operating conditions of solid-state fermentation were optimized using the method of full factorial design with incubation temperature ranging between 25 C and 35 C moisture content between 40 and 60 and inoculum size between 1.6 x 106 spores/mL and 1.4 x 107 spores/mL. Optimizing the solid-state fermentation conditions appeared crucial to minimize the sample used in this experimental design and determine the significant correlation between the operating conditions. A relatively high maximal pectinase production of 27 UmL-1 was attained at 35 C of incubation 60 of moisture content and 1.6 x 106 spores/mL of inoculum size with a relatively low amount of substrate (5 g). Given that the production of pectinase with other substrates (e.g. pineapple waste lemon peel cassava waste and wheat bran) generally ranges between 3 U/mL and 16 U/mL (Abdullah et al. 2018; Handa et al. 2016; Melnichuk et al. 2020; Thangaratham and Manimegalai 2014; Salim et al. 2017) thus the yield of pectinase derived from the banana peel in this study (27 U/mL) was considered moderately high. The findings of this study indicated that the biomass of banana peel would be a potential substrate for pectinase production via the solid-state fermentation of A. niger.
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Abstract
In this study the biomass of banana peel was used to produce pectinase via optimization of solid-state fermentation conditions of the filamentous fungi Aspergillus nigeA. niger). The operating conditions of solid-state fermentation were optimized using the method of full factorial design with incubation temperature ranging between 25 C and 35 C moisture content between 40 and 60 and inoculum size between 1.6 x 106 spores/mL and 1.4 x 107 spores/mL. Optimizing the solid-state fermentation conditions appeared crucial to minimize the sample used in this experimental design and determine the significant correlation between the operating conditions. A relatively high maximal pectinase production of 27 UmL-1 was attained at 35 C of incubation 60 of moisture content and 1.6 x 106 spores/mL of inoculum size with a relatively low amount of substrate (5 g). Given that the production of pectinase with other substrates (e.g. pineapple waste lemon peel cassava waste and wheat bran) generally ranges between 3 U/mL and 16 U/mL (Abdullah et al. 2018; Handa et al. 2016; Melnichuk et al. 2020; Thangaratham and Manimegalai 2014; Salim et al. 2017) thus the yield of pectinase derived from the banana peel in this study (27 U/mL) was considered moderately high. The findings of this study indicated that the biomass of banana peel would be a potential substrate for pectinase production via the solid-state fermentation of A. niger.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Bananas |
AGROVOC Term: | Peel |
AGROVOC Term: | Biomass |
AGROVOC Term: | Aspergillus niger |
AGROVOC Term: | Optimization methods |
AGROVOC Term: | Solid state fermentation |
AGROVOC Term: | Pectinase |
AGROVOC Term: | Agricultural waste management |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:55 |
URI: | http://webagris.upm.edu.my/id/eprint/10276 |
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