Cardioprotective effects of Gynura procumbens extract on oxidative status and myocardial injury in rats with isoproterenol-induced myocardial infarction

Das S., . and N. Abd Ghafar, . and S. Zainalabidin, . and Z. Jubri, . and S. A. Syed Abd Halim, . Cardioprotective effects of Gynura procumbens extract on oxidative status and myocardial injury in rats with isoproterenol-induced myocardial infarction. pp. 1223-1232. ISSN 22317546

Gynura procumbens (GP) grows abundantly in Southeast Asia. The present work was conducted to investigate the cardioprotective potential of ethanol extract of GP on cardiac markers antioxidant levels and histopathology of isoproterenol-induced myocardial infarction (MI). A total of 36 adult Sprague-Dawley rats were randomly divided into six groups. Treatments were given via oral gavage for 28 days: two groups were given normal saline 0.9; two groups were given GP250 mg/kg/day- and two groups were given GP500 mg/kg/day. On day 27 and 28 MI was induced with a subcutaneous injection of 85 mg/kg isoproterenol. The rats were sacrificed 48h after the 1st injection. Cardiac markers lipid peroxidation oxidative status and histopathological analyses were evaluated. Isoproterenol significantly increased the levels of troponin T creatine kinase MB isoenzyme (CKMB) lactate dehydrogenase (LDH) and malondialdehyde (MDA) whereas the level of superoxide dismutase (SOD) catalase and glutathione peroxidase were significantly decreased. In addition the histopathological findings showed a necrosis of the myocardium as evidenced by neutrophil infiltration and interstitial oedema with acceleration of apoptosis in MI. Interestingly treatment with GP restored the levels of troponin T LDH MDA SOD and catalase significantly. Moreover GP preserved the myocardial architecture while decreasing both necrosis and apoptosis. GP has the potential to limit myocardial injury after MI and this is most likely achieved through its modulation of antioxidant enzyme activities.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/28%20(06)%202021/DONE%2...

Abstract

Gynura procumbens (GP) grows abundantly in Southeast Asia. The present work was conducted to investigate the cardioprotective potential of ethanol extract of GP on cardiac markers antioxidant levels and histopathology of isoproterenol-induced myocardial infarction (MI). A total of 36 adult Sprague-Dawley rats were randomly divided into six groups. Treatments were given via oral gavage for 28 days: two groups were given normal saline 0.9; two groups were given GP250 mg/kg/day- and two groups were given GP500 mg/kg/day. On day 27 and 28 MI was induced with a subcutaneous injection of 85 mg/kg isoproterenol. The rats were sacrificed 48h after the 1st injection. Cardiac markers lipid peroxidation oxidative status and histopathological analyses were evaluated. Isoproterenol significantly increased the levels of troponin T creatine kinase MB isoenzyme (CKMB) lactate dehydrogenase (LDH) and malondialdehyde (MDA) whereas the level of superoxide dismutase (SOD) catalase and glutathione peroxidase were significantly decreased. In addition the histopathological findings showed a necrosis of the myocardium as evidenced by neutrophil infiltration and interstitial oedema with acceleration of apoptosis in MI. Interestingly treatment with GP restored the levels of troponin T LDH MDA SOD and catalase significantly. Moreover GP preserved the myocardial architecture while decreasing both necrosis and apoptosis. GP has the potential to limit myocardial injury after MI and this is most likely achieved through its modulation of antioxidant enzyme activities.

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Item Type:	Article
AGROVOC Term:	Oxidative stress
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Rats
AGROVOC Term:	Antioxidants
AGROVOC Term:	Sampling
AGROVOC Term:	Experimental design
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Preservation
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10311

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