An improved method for the purification and structural analysis of rubropunctatin from red yeast glutinous rice

Hu J. W., . and Fu R. Y., . and Zhou Y. B., . and Wei D. M., . and Sui T., . and He L. Y. Z., . and Shen M. Y., . and Xu L., . and Cao C., . An improved method for the purification and structural analysis of rubropunctatin from red yeast glutinous rice. pp. 1276-1284. ISSN 22317546

An improved method for the separation and purification of rubropunctatin from red yeast glutinous rice was developed. In this method silica gel column chromatography and thin layer chromatography were used to separate the Monascus pigments. Pure methanol and a mixture of ethyl acetate and methanol (13:7 v/v) were selected as the eluent and running solvent respectively. When compared with previously reported methods the developed method required fewer types of solvents and could be suitable for large-scale laboratory production. The extracted pigment was analysed by ultra-high performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS) and nuclear magnetic resonance (NMR) (H NMR C NMR). Structural analysis revealed a molecular mass of 353.2 m/z M�H� and a structure including an azaphilone body with two side chains. This structure was consistent with that reported for rubropunctatin. Therefore the improved purification method reported herein could efficiently be used for the extraction of rubropunctatin from red yeast glutinous rice.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/28%20(06)%202021/DONE%2...

Abstract

An improved method for the separation and purification of rubropunctatin from red yeast glutinous rice was developed. In this method silica gel column chromatography and thin layer chromatography were used to separate the Monascus pigments. Pure methanol and a mixture of ethyl acetate and methanol (13:7 v/v) were selected as the eluent and running solvent respectively. When compared with previously reported methods the developed method required fewer types of solvents and could be suitable for large-scale laboratory production. The extracted pigment was analysed by ultra-high performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS) and nuclear magnetic resonance (NMR) (H NMR C NMR). Structural analysis revealed a molecular mass of 353.2 m/z M�H� and a structure including an azaphilone body with two side chains. This structure was consistent with that reported for rubropunctatin. Therefore the improved purification method reported herein could efficiently be used for the extraction of rubropunctatin from red yeast glutinous rice.

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Item Type:	Article
AGROVOC Term:	Yeasts
AGROVOC Term:	Laboratory experimentation
AGROVOC Term:	Sampling
AGROVOC Term:	analysis
AGROVOC Term:	Spectroscopy
AGROVOC Term:	Purification
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10317

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