Detection of PirA/B toxin genes for acute hepatopancreatic necrosis disease (AHPND) and Vibrio parahaemolyticus in Penaeus vannamei culture from major white shrimp producing farms in Malaysia

Bakar Padilah, . and Rohaiza Asmini Yahya, . and Han Ming Gan, . and Wan Ahmad Wan Rozana, . and Wan Muhammad Hazim Wan Sajiri, . and Beng Chu Kua, . Detection of PirA/B toxin genes for acute hepatopancreatic necrosis disease (AHPND) and Vibrio parahaemolyticus in Penaeus vannamei culture from major white shrimp producing farms in Malaysia. pp. 171-186. ISSN 1511-3701

The acute hepatopancreatic necrosis disease (AHPND) epidemic from 2010 to 2013 significantly affected white shrimp (Penaeus vannamei) production in Malaysia. This study aims to determine the status of AHPND in P. vannamei culture from detecting PirA/B toxin genes in hepatopancreas tissues and isolation of Vibrio parahaemolyticus for identification of pathogenic strain from major white shrimp producing farms in Malaysia. Bacteria from the hepatopancreas organ were cultured on tryptic soy agar and identified using API 20 NE (bioMrieux France) for Vibrio species. Confirmation of PirA/B toxin genes in hepatopancreas and V. parahaemolyticus isolates were determined by polymerase chain reaction (PCR). Twenty-three V. parahaemolyticus isolates were identified from 7.7 of the analysed samples. Fourteen (14) (4.7) were detected with PirA/B toxin genes from districts of Johor such as Batu Pahat (1) and Kota Tinggi (8) Alor Setar Kedah (3) Tawau Sabah (1) and Kuching Sarawak (1). In contrast the other nine isolates (3) contained only the plasmid. Genomic and phylogenetic tree analysis of four V. parahaemolyticus isolates carrying PirA/B toxin genes from this study showed that only one strain (Vp14) harbours the PirA/B complete genes in addition to displaying full sequence homology and coverage to the pVA1 plasmid. In contrast other strains (AAT22 IKK3 and PK3) displayed partial sequence homology of plasmid harbouring key genes associated with conjugative transfer function but not the plasmid segments containing PirA/B toxin genes. Hence this study showed that six farms were negative from AHPND. In contrast four farms were positive with PirA/B toxin genes in juveniles from Pekan Pahang (26.7) Kuching and Sarikei Sarawak (10 respectively) and Alor Setar Kedah (3.3).

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Abstract

The acute hepatopancreatic necrosis disease (AHPND) epidemic from 2010 to 2013 significantly affected white shrimp (Penaeus vannamei) production in Malaysia. This study aims to determine the status of AHPND in P. vannamei culture from detecting PirA/B toxin genes in hepatopancreas tissues and isolation of Vibrio parahaemolyticus for identification of pathogenic strain from major white shrimp producing farms in Malaysia. Bacteria from the hepatopancreas organ were cultured on tryptic soy agar and identified using API 20 NE (bioMrieux France) for Vibrio species. Confirmation of PirA/B toxin genes in hepatopancreas and V. parahaemolyticus isolates were determined by polymerase chain reaction (PCR). Twenty-three V. parahaemolyticus isolates were identified from 7.7 of the analysed samples. Fourteen (14) (4.7) were detected with PirA/B toxin genes from districts of Johor such as Batu Pahat (1) and Kota Tinggi (8) Alor Setar Kedah (3) Tawau Sabah (1) and Kuching Sarawak (1). In contrast the other nine isolates (3) contained only the plasmid. Genomic and phylogenetic tree analysis of four V. parahaemolyticus isolates carrying PirA/B toxin genes from this study showed that only one strain (Vp14) harbours the PirA/B complete genes in addition to displaying full sequence homology and coverage to the pVA1 plasmid. In contrast other strains (AAT22 IKK3 and PK3) displayed partial sequence homology of plasmid harbouring key genes associated with conjugative transfer function but not the plasmid segments containing PirA/B toxin genes. Hence this study showed that six farms were negative from AHPND. In contrast four farms were positive with PirA/B toxin genes in juveniles from Pekan Pahang (26.7) Kuching and Sarikei Sarawak (10 respectively) and Alor Setar Kedah (3.3).

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Item Type:	Article
AGROVOC Term:	Penaeus vannamei
AGROVOC Term:	Aquaculture
AGROVOC Term:	Ponds
AGROVOC Term:	Bacterial diseases
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Vibrio parahaemolyticus
AGROVOC Term:	Acute disease
AGROVOC Term:	Disease prevalence
AGROVOC Term:	genomics
AGROVOC Term:	Farm management
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10362

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