Isolation and molecular identification of a siderophore producing bacterium and its antagonistic effect against Fusarium oxysporum f. sp. cubense Tropical Race 4

Siti Norhidayah Othman, . and Seok Christina Yien Yong, . and Nur Ain Izzati Mohd Zainudin, . and Muskhazli Mustafa, . and Yu Rou Ch’ng, . Isolation and molecular identification of a siderophore producing bacterium and its antagonistic effect against Fusarium oxysporum f. sp. cubense Tropical Race 4. pp. 187-206. ISSN 1511-3701

Bananas are one of the worlds most consumed fruits. Developing countries in the Global South depend on bananas for food security and livelihoods. Still the banana industry also drives a multinational trade worth billions of US dollars. In addition banana plants also hold cultural and religious significances in many Asian countries. However banana production faces several challenges and one of the major issues is the Fusarium wilt disease caused by the fungus Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4). This disease threatens numerous banana cultivars including the Cavendish the most traded banana cultivar. Therefore the objective of this study was to find effective measures to control the spread of this disease through antagonistic soil bacteria. This study isolated 14 fluorescent siderophore-producing bacteria with in vitro inhibition rates of 21.73-50.38 against Foc TR4 from the soil surrounding banana plants. Most of the isolates were identified as Pseudomonas spp. via 16S rRNA sequencing and phylogenetic analysis based on 16s rRNA sequences showed clustering of isolates congruent with the results of similarity searches. Three isolates that exhibited relatively higher antagonistic activity against Fusarium oxysporum f. sp. cubense compared to other isolates were JBAA132 (50.38) K2B131 (46.28) and KTP231 (45.38). Isolate JBAA132 displayed differences in biochemical characteristics compared to its closest match (Pseudomonas aeruginosa type strain DSM50071). It also demonstrated nitrogen-fixing and phosphate solubilising properties common to plant growth promoters. Therefore isolate JBAA132 may be further explored as a potential biocontrol agent in curbing the spread of Foc TR4.

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Abstract

Bananas are one of the worlds most consumed fruits. Developing countries in the Global South depend on bananas for food security and livelihoods. Still the banana industry also drives a multinational trade worth billions of US dollars. In addition banana plants also hold cultural and religious significances in many Asian countries. However banana production faces several challenges and one of the major issues is the Fusarium wilt disease caused by the fungus Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4). This disease threatens numerous banana cultivars including the Cavendish the most traded banana cultivar. Therefore the objective of this study was to find effective measures to control the spread of this disease through antagonistic soil bacteria. This study isolated 14 fluorescent siderophore-producing bacteria with in vitro inhibition rates of 21.73-50.38 against Foc TR4 from the soil surrounding banana plants. Most of the isolates were identified as Pseudomonas spp. via 16S rRNA sequencing and phylogenetic analysis based on 16s rRNA sequences showed clustering of isolates congruent with the results of similarity searches. Three isolates that exhibited relatively higher antagonistic activity against Fusarium oxysporum f. sp. cubense compared to other isolates were JBAA132 (50.38) K2B131 (46.28) and KTP231 (45.38). Isolate JBAA132 displayed differences in biochemical characteristics compared to its closest match (Pseudomonas aeruginosa type strain DSM50071). It also demonstrated nitrogen-fixing and phosphate solubilising properties common to plant growth promoters. Therefore isolate JBAA132 may be further explored as a potential biocontrol agent in curbing the spread of Foc TR4.

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Item Type:	Article
AGROVOC Term:	Biological control
AGROVOC Term:	Biocontrol
AGROVOC Term:	Fusarium oxysporum
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Siderophores
AGROVOC Term:	Antagonistic fungi
AGROVOC Term:	Panama disease
AGROVOC Term:	Pseudomonas
AGROVOC Term:	Pathogenicity
AGROVOC Term:	Plant growth promoting bacteria
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10363

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