Sequencing analysis of partial N gene of feline Morbillivirus from Malaysia

Sheau Wei Tan, . and Siti Tasnim Makhtar, . and Abdul Rahman Omar, . and Farina Mustaffa Kamal, . Sequencing analysis of partial N gene of feline Morbillivirus from Malaysia. pp. 337-349. ISSN 1511-3701

Feline morbillivirus (FeMV) is a new emerging virus of domestic cats categorized under the genus of (Morbillivirus associated with chronic kidney disease (CKD). The origin of the virus is yet to be determined and whether it is caused by a spill-over event from wildlife or domestic cats remains speculative. Recombination event has been reported in FeMV isolate found in Japan; therefore characterization of FeMV strains isolated in Malaysia (i.e. FeMV-Malaysia isolates) may provide some insight thus adding some information on the viral evolution of FeMV. Therefore this study aims to conduct a phylogenetic analysis and assess any genetic changes in the N gene of FeMV-Malaysia isolates. Through sequencing of N gene of seven isolates using three overlapping primer sets the sequences spanning approximately 1.5kb of FeMV-N gene were obtained. DNA sequencing nucleotide sequences amino acid residues alignments and phylogenetic analysis were performed. A nucleotide sequence alignment was also performed to compare the isolates obtained from two previous studies. From the alignment mentioned above there were 19 variable sites of which there were absence of amino acid changes except for isolate UPM210 at position 806 and isolate UPM315 at position 823. Furthermore protein alignment was done to compare FeMV-Malaysia isolates with FeMV strains from other countries along with other morbillivirus-related isolates. From one of the conserved regions located within the N gene similar amino acid sequences were detected across different morbilliviruses. Lastly from the phylogenetic tree it was illustrated that all partial FeMV-N gene Malaysia isolates sequenced in this study were clustered together in the same clade whereby these FeMV-N genes Malaysia isolates shared a common ancestor with isolates from Japan (SS3 MiJP003 ChJP073) and Thailand (Thai-U16 CTL16. CTL43).

Full text available from: Official URL: http://www.pertanika.upm.edu.my/pjtas/browse/regul...

Abstract

Feline morbillivirus (FeMV) is a new emerging virus of domestic cats categorized under the genus of (Morbillivirus associated with chronic kidney disease (CKD). The origin of the virus is yet to be determined and whether it is caused by a spill-over event from wildlife or domestic cats remains speculative. Recombination event has been reported in FeMV isolate found in Japan; therefore characterization of FeMV strains isolated in Malaysia (i.e. FeMV-Malaysia isolates) may provide some insight thus adding some information on the viral evolution of FeMV. Therefore this study aims to conduct a phylogenetic analysis and assess any genetic changes in the N gene of FeMV-Malaysia isolates. Through sequencing of N gene of seven isolates using three overlapping primer sets the sequences spanning approximately 1.5kb of FeMV-N gene were obtained. DNA sequencing nucleotide sequences amino acid residues alignments and phylogenetic analysis were performed. A nucleotide sequence alignment was also performed to compare the isolates obtained from two previous studies. From the alignment mentioned above there were 19 variable sites of which there were absence of amino acid changes except for isolate UPM210 at position 806 and isolate UPM315 at position 823. Furthermore protein alignment was done to compare FeMV-Malaysia isolates with FeMV strains from other countries along with other morbillivirus-related isolates. From one of the conserved regions located within the N gene similar amino acid sequences were detected across different morbilliviruses. Lastly from the phylogenetic tree it was illustrated that all partial FeMV-N gene Malaysia isolates sequenced in this study were clustered together in the same clade whereby these FeMV-N genes Malaysia isolates shared a common ancestor with isolates from Japan (SS3 MiJP003 ChJP073) and Thailand (Thai-U16 CTL16. CTL43).

[error in script]

Item Type:	Article
AGROVOC Term:	Morbillivirus
AGROVOC Term:	Cats
AGROVOC Term:	Kidney diseases
AGROVOC Term:	Genes
AGROVOC Term:	Phylogeny
AGROVOC Term:	animal genetics
AGROVOC Term:	DNA sequence
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10372

Actions (login required)

View Item