Citation
Faizul Fikri M. Y., . and Syamsiah Aini S., . and Ong G. H., . and Maizatul Z., . and Muhammad Redzuan S., . and Mohammad Jiha R., . and Leow B. L., . Molecular characterization of fowl adenoviruses isolated from chickens in Malaysia. pp. 59-67. ISSN 2180-3897
Abstract
Recently Fowl Adenovirus (FAdV) cases have been reported in many countries worldwide. FAdV is a contagious agent associated with inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) in chickens. It belongs to the Aviadenovirus genus of the family Adenoviridae. The virus is classified into five species (A to E) and further divided into 12 serotypes. Depending on the serotypes they have diverse characteristics of virus that can either be pathogenic or nonpathogenic strain. From the viewpoint of epidemiological as well as vaccine development it is very important to detect FAdV strains. Previous studies have been conducted on molecular research but the continuity of this study in Malaysia has been limited. This study aims to identify the serotype classification of five Malaysian FAdV isolates obtained from field outbreaks during 2017-2019. In this study polymerase chain reactions (PCR) were conducted based on Hexon gene. Results from the nucleotide sequence analysis discovered that the five isolates showed high similarity with FAdV-8b strains. High bootstrap values in phylogenetic analysis supported the clustering of the Malaysian FAdVs isolates into FAdVs species E. Consequently the result of this study contributed important information on the epidemiology and culminated in the importance of control strategies against FAdV infection in Malaysia.
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Abstract
Recently Fowl Adenovirus (FAdV) cases have been reported in many countries worldwide. FAdV is a contagious agent associated with inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) in chickens. It belongs to the Aviadenovirus genus of the family Adenoviridae. The virus is classified into five species (A to E) and further divided into 12 serotypes. Depending on the serotypes they have diverse characteristics of virus that can either be pathogenic or nonpathogenic strain. From the viewpoint of epidemiological as well as vaccine development it is very important to detect FAdV strains. Previous studies have been conducted on molecular research but the continuity of this study in Malaysia has been limited. This study aims to identify the serotype classification of five Malaysian FAdV isolates obtained from field outbreaks during 2017-2019. In this study polymerase chain reactions (PCR) were conducted based on Hexon gene. Results from the nucleotide sequence analysis discovered that the five isolates showed high similarity with FAdV-8b strains. High bootstrap values in phylogenetic analysis supported the clustering of the Malaysian FAdVs isolates into FAdVs species E. Consequently the result of this study contributed important information on the epidemiology and culminated in the importance of control strategies against FAdV infection in Malaysia.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Fowl pest virus |
AGROVOC Term: | Chickens |
AGROVOC Term: | Isolation techniques |
AGROVOC Term: | Adenoviridae |
AGROVOC Term: | Aviadenovirus |
AGROVOC Term: | Serotypes |
AGROVOC Term: | Polymerase chain reaction |
AGROVOC Term: | Classification |
AGROVOC Term: | Infection |
AGROVOC Term: | DNA sequence |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:55 |
URI: | http://webagris.upm.edu.my/id/eprint/10376 |
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