Molecular phylogenetics of Newcastle disease virus isolated from chickens in 2019

Faizul Fikri M. Y., . and Leow B. L., . and Muhammad Redzwa S., . and Faizah Hanim M. S., . and Syamsiah Aini S., . Molecular phylogenetics of Newcastle disease virus isolated from chickens in 2019. pp. 51-58. ISSN 2180-3897

Newcastle disease (ND) is an economically important contagious poultry viral disease reported across the globe. No recent reports on ND circulating in Malaysia. Therefore the aim of the study is to characterize 16 Newcastle disease viruses (NDVs) isolated from chickens in Malaysia in the year of 2019. All isolates were genotypically analyzed using reverse transcription-polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Analysis of the F protein cleavage sites deduced amino acid sequences revealed that from the Newcastle disease virus (NDV) isolates three of them were virulent with two different motifs of 112RRQKRF117 and 12RRRKRF117 while other isolates were avirulent. Phylogenetic analysis demonstrated that three isolates were grouped in genotype VII five in genotype I while eight in genotype II. All genotype VII isolates were clustered under sub-genotype VII.2 (VIIh and VIIi) which is the same strain causing previous outbreaks in Malaysia. Therefore findings in this study demonstrated that there is no new introduction of NDV genotypes in Malaysia. However farms should implement biosecurity measures at strict level as well as executing continuous monitoring and surveillance of the disease as these implementations would help them to conduct proper preventive measures and control of panzootic viruses in future.

Full text available from: Official URL: http://research.dvs.gov.my/portal/wp-content/uploa...

Abstract

Newcastle disease (ND) is an economically important contagious poultry viral disease reported across the globe. No recent reports on ND circulating in Malaysia. Therefore the aim of the study is to characterize 16 Newcastle disease viruses (NDVs) isolated from chickens in Malaysia in the year of 2019. All isolates were genotypically analyzed using reverse transcription-polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Analysis of the F protein cleavage sites deduced amino acid sequences revealed that from the Newcastle disease virus (NDV) isolates three of them were virulent with two different motifs of 112RRQKRF117 and 12RRRKRF117 while other isolates were avirulent. Phylogenetic analysis demonstrated that three isolates were grouped in genotype VII five in genotype I while eight in genotype II. All genotype VII isolates were clustered under sub-genotype VII.2 (VIIh and VIIi) which is the same strain causing previous outbreaks in Malaysia. Therefore findings in this study demonstrated that there is no new introduction of NDV genotypes in Malaysia. However farms should implement biosecurity measures at strict level as well as executing continuous monitoring and surveillance of the disease as these implementations would help them to conduct proper preventive measures and control of panzootic viruses in future.

[error in script]

Item Type:	Article
AGROVOC Term:	Newcastle disease virus
AGROVOC Term:	Chickens
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Reverse transcription
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	RNA sequence
AGROVOC Term:	Biosecurity
AGROVOC Term:	Disease surveillance
AGROVOC Term:	Disease prevention
AGROVOC Term:	Poultry farming
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10377

Actions (login required)

View Item