Assessment of bacteriological quality and Escherichia coli O157: H7 in the ready-to-eat street food

P. Arkappan, . and M.N.S.S. Hameed, . and R.J. Sreedharam, . and P. Sandrasaigaran, . and S. R. Latchumaya, . Assessment of bacteriological quality and Escherichia coli O157: H7 in the ready-to-eat street food. pp. 98-105. ISSN 2550-2166

Ready-to-eat (RTE) street foods are usually prepared well in advance (4-8 hrs) before the sale. Owning to minimal cooking procedures cross-contaminations and abused holding temperature and time there is a high probability for deteriorating microbiological quality in these foods. Thus this study was aimed to assess the bacteriological quality of RTE foods sampled from night markets and street stalls based on the guidelines provided by the Public Health Laboratory Service. A total of fifty samples (category 2 3 and 4) were evaluated for aerobic colony count total coliform and E. coli. The pathogenic strain E. coli O157: H7 were examined using the multiplex PCR technique. It was noticed that category 3 and 4 RTE street foods were largely unsatisfactory for the coliform (10�´ CFU/ mL) and E. coli count (100 CFU/mL). In opposite category 2 RTE street foods were unsatisfactory for the aerobic colony count (10� CFU/mL) and E. coli (100 CFU/ mL). However there was no E. coli O157: H7 or Shiga-toxin producing bacteria reported in this study. The statistical analysis showed that in overall category 3 and 4 RTE street foods were significantly at worsening bacteriological quality compared to category 2. Strict legal enforcement and amending the existing rules are needed to improve the quality of RTE street foods sold in the night markets and street stalls.

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Abstract

Ready-to-eat (RTE) street foods are usually prepared well in advance (4-8 hrs) before the sale. Owning to minimal cooking procedures cross-contaminations and abused holding temperature and time there is a high probability for deteriorating microbiological quality in these foods. Thus this study was aimed to assess the bacteriological quality of RTE foods sampled from night markets and street stalls based on the guidelines provided by the Public Health Laboratory Service. A total of fifty samples (category 2 3 and 4) were evaluated for aerobic colony count total coliform and E. coli. The pathogenic strain E. coli O157: H7 were examined using the multiplex PCR technique. It was noticed that category 3 and 4 RTE street foods were largely unsatisfactory for the coliform (10�´ CFU/ mL) and E. coli count (100 CFU/mL). In opposite category 2 RTE street foods were unsatisfactory for the aerobic colony count (10� CFU/mL) and E. coli (100 CFU/ mL). However there was no E. coli O157: H7 or Shiga-toxin producing bacteria reported in this study. The statistical analysis showed that in overall category 3 and 4 RTE street foods were significantly at worsening bacteriological quality compared to category 2. Strict legal enforcement and amending the existing rules are needed to improve the quality of RTE street foods sold in the night markets and street stalls.

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Item Type:	Article
AGROVOC Term:	Bacteriological analysis
AGROVOC Term:	Escherichia coli
AGROVOC Term:	Street foods
AGROVOC Term:	Street markets
AGROVOC Term:	Ready meals
AGROVOC Term:	Stalls
AGROVOC Term:	Microbiological contamination
AGROVOC Term:	Bacteriological analysis
AGROVOC Term:	PCR
AGROVOC Term:	Foodborne diseases
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10394

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