Quantitation and optimization of -carotene and -tocopherol in emulsion prototype with reversed-phase chromatography

Rohmah M., . and Rahmadi A., . and Yanti I., . and Jannah S. M., . and Bohari., . and Supratman U., . Quantitation and optimization of -carotene and -tocopherol in emulsion prototype with reversed-phase chromatography. pp. 290-297. ISSN 2550-2166

Emulsion products that are rich in pro-vitamin A vitamin E and antioxidants have been successfully formulated from red palm oil (FO-MSM) pumpkin (SLK) and dragon fruit (SBN). This study was aimed to determine the concentrations of -carotene and tocopherol in the prototype products of FO-MSM SLK and SBN by reverse phase chromatography and to determine the optimal deodorization time for FO-MSM on carotene and -tocopherol levels. Optimal conditions for analysis of samples was with the mobile phase methyl dichloride: methanol: acetonitrile (2: 1: 3). The injection volume was 10 L for -carotene and 20 L for -tocopherol. Isocratic elution was carried out at a flow rate of 1.0 mL/min at room temperature (252C). The mobile phase was first eliminated for 45 mins. The -carotene and -tocopherol peaks were identified by matching the peak retention times (Rt) of -carotene and -tocopherol in the sample with the standard Rt of -carotene and -tocopherol. The reverse-phase chromatography successfully determined -carotene and -tocopherol in prototype products of FO-MSM SLK and SBN. The developed emulsion prototype had 2044.5196.1 ppm of -carotene and 38.80.4 ppm of -tocopherol. The content of -carotene in the developed emulsion prototype was more than tenfold increase from the original prototype without sacrificing the product's taste. -carotene showed no significant difference in reducing the deodorization time from 5 hrs to 1 hr while the 2.5-fold increase of the -tocopherol content was observed. Therefore the optimal deodorization time for FO-MSM was determined based on the value of -tocopherol and not by -carotene. RP-18 was successfully used to determine -carotene and -tocopherol in FO-MSM SLK and SBN emulsion prototype products.

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Abstract

Emulsion products that are rich in pro-vitamin A vitamin E and antioxidants have been successfully formulated from red palm oil (FO-MSM) pumpkin (SLK) and dragon fruit (SBN). This study was aimed to determine the concentrations of -carotene and tocopherol in the prototype products of FO-MSM SLK and SBN by reverse phase chromatography and to determine the optimal deodorization time for FO-MSM on carotene and -tocopherol levels. Optimal conditions for analysis of samples was with the mobile phase methyl dichloride: methanol: acetonitrile (2: 1: 3). The injection volume was 10 L for -carotene and 20 L for -tocopherol. Isocratic elution was carried out at a flow rate of 1.0 mL/min at room temperature (252C). The mobile phase was first eliminated for 45 mins. The -carotene and -tocopherol peaks were identified by matching the peak retention times (Rt) of -carotene and -tocopherol in the sample with the standard Rt of -carotene and -tocopherol. The reverse-phase chromatography successfully determined -carotene and -tocopherol in prototype products of FO-MSM SLK and SBN. The developed emulsion prototype had 2044.5196.1 ppm of -carotene and 38.80.4 ppm of -tocopherol. The content of -carotene in the developed emulsion prototype was more than tenfold increase from the original prototype without sacrificing the product's taste. -carotene showed no significant difference in reducing the deodorization time from 5 hrs to 1 hr while the 2.5-fold increase of the -tocopherol content was observed. Therefore the optimal deodorization time for FO-MSM was determined based on the value of -tocopherol and not by -carotene. RP-18 was successfully used to determine -carotene and -tocopherol in FO-MSM SLK and SBN emulsion prototype products.

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Item Type:	Article
AGROVOC Term:	Tocopherols
AGROVOC Term:	Carotenes
AGROVOC Term:	Reverse transcription
AGROVOC Term:	Chromatography
AGROVOC Term:	Emulsions
AGROVOC Term:	Carotenoids
AGROVOC Term:	Tocotrienols
AGROVOC Term:	Crude oil
AGROVOC Term:	Deodorization
AGROVOC Term:	Prototypes
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10415

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