Antiradical activity total phenolic and total flavonoids extract and fractions of pumpkin (Cucurbita moshata Duch) leaves

Sartinah A., . and Ruslin, . and Buton S., . and Sabarudin, . and Zubaydah W. O. S., . and Yamin, . Antiradical activity total phenolic and total flavonoids extract and fractions of pumpkin (Cucurbita moshata Duch) leaves. pp. 348-353. ISSN 2550-2166

Pumpkin (Cucurbita moshata Duch) is one of the Cucurbitaceae plants which traditionally used to treat skin diseases measles jaundice insomnia cancer and enhances endurance. Therefore it was necessary to explore the potential of pumpkin leaves as antiradical. This research aim was to examine the antiradical activity and total phenolic and total flavonoids of pumpkin leaves extract and its fractions using the DPPH method and determined the phenolic and flavonoid contents. Pumpkin leave powder was extracted with methanol. Furthermore water was added into methanol extract and be partitioned using n-hexane and ethyl acetate to obtain n-hexane ethyl acetate and water fractions. The antiradical activities of pumpkin leave extract and fractions were determined using DPPH (2 2-diphenyl-1-picryhidrazyl) method. Ethyl acetate fraction obtained higher antiradical activities (IC‚‚ 6.7370.196 g/mL). Correlation of total phenolic and flavonoid contents to inhibit DPPH radical showed that phenolic and flavonoid contents on pumpkin leaves could be inhibited DPPH radical R 0.8994 and R 0.9061 respectively. Extracts and fraction pumpkin leaves show strong antiradical activity with DPPH methods so their potential as antiradical can developed and can be used as a functional food.

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Abstract

Pumpkin (Cucurbita moshata Duch) is one of the Cucurbitaceae plants which traditionally used to treat skin diseases measles jaundice insomnia cancer and enhances endurance. Therefore it was necessary to explore the potential of pumpkin leaves as antiradical. This research aim was to examine the antiradical activity and total phenolic and total flavonoids of pumpkin leaves extract and its fractions using the DPPH method and determined the phenolic and flavonoid contents. Pumpkin leave powder was extracted with methanol. Furthermore water was added into methanol extract and be partitioned using n-hexane and ethyl acetate to obtain n-hexane ethyl acetate and water fractions. The antiradical activities of pumpkin leave extract and fractions were determined using DPPH (2 2-diphenyl-1-picryhidrazyl) method. Ethyl acetate fraction obtained higher antiradical activities (IC‚‚ 6.7370.196 g/mL). Correlation of total phenolic and flavonoid contents to inhibit DPPH radical showed that phenolic and flavonoid contents on pumpkin leaves could be inhibited DPPH radical R 0.8994 and R 0.9061 respectively. Extracts and fraction pumpkin leaves show strong antiradical activity with DPPH methods so their potential as antiradical can developed and can be used as a functional food.

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Item Type:	Article
AGROVOC Term:	Pumpkins
AGROVOC Term:	Cucurbita moschata
AGROVOC Term:	Diphenyl
AGROVOC Term:	Phenolic acids
AGROVOC Term:	Flavonoids
AGROVOC Term:	Cucurbitaceae
AGROVOC Term:	Leaves
AGROVOC Term:	Extraction
AGROVOC Term:	Functional foods
AGROVOC Term:	Free radicals
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10422

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