Formulation of alternative media for fungal growth and its application as agarwood-inducing agent in Aquilaria trees

Noor Baity, . and Wan Muhammad Azrul W. A., . and Hazandy A. H., . and Mohd Farid A., . and Rozihawati Z., . and Zaiton S., . and Sheriza M. R., . Formulation of alternative media for fungal growth and its application as agarwood-inducing agent in Aquilaria trees. pp. 127-132. ISSN 0128-1283

This study investigated the growth performance of Fusarium proliferatum isolated from agarwood on different types of plated media prepared with liquid broth extracts of locally available agricultural products. The potential of F. proliferatum in selected media to induce agarwood formation in Aquilaria trees was also assessed. A total of 27 media formulations were evaluated as alternative media to potato dextrose agar (PDA) for F. proliferatum. The effects of culture media on the diameter growth of fungal mycelium were significant (F 6.70 df 26 p 0.01). The best growth was recorded on 50 soybean followed by PDA and 70 kidney bean with mean mycelium diameters of 7.00 0.00 6.63 0.83 and 6.50 0.06 cm respectively. In the field trial F. proliferatum in all three media successfully induced agarwood formation on A. malaccensis and A. crassna with significant discoloration zones compared with the uninoculated control trees (p 0.05) after five months. Fifty percent soybean in combination with F. proliferatum induced the largest discoloration zone indicating potential as a cheaper and more accessible alternative to PDA. These results suggest that the combination of a chemical inducer in the form of a liquid media formulation and a biological inducer in this case an endophytic fungus could successfully induce agarwood formation in Aquilaria trees.

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Abstract

This study investigated the growth performance of Fusarium proliferatum isolated from agarwood on different types of plated media prepared with liquid broth extracts of locally available agricultural products. The potential of F. proliferatum in selected media to induce agarwood formation in Aquilaria trees was also assessed. A total of 27 media formulations were evaluated as alternative media to potato dextrose agar (PDA) for F. proliferatum. The effects of culture media on the diameter growth of fungal mycelium were significant (F 6.70 df 26 p 0.01). The best growth was recorded on 50 soybean followed by PDA and 70 kidney bean with mean mycelium diameters of 7.00 0.00 6.63 0.83 and 6.50 0.06 cm respectively. In the field trial F. proliferatum in all three media successfully induced agarwood formation on A. malaccensis and A. crassna with significant discoloration zones compared with the uninoculated control trees (p 0.05) after five months. Fifty percent soybean in combination with F. proliferatum induced the largest discoloration zone indicating potential as a cheaper and more accessible alternative to PDA. These results suggest that the combination of a chemical inducer in the form of a liquid media formulation and a biological inducer in this case an endophytic fungus could successfully induce agarwood formation in Aquilaria trees.

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Item Type:	Article
AGROVOC Term:	Fusarium proliferatum
AGROVOC Term:	endophytic
AGROVOC Term:	Thymelaeaceae
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Culture media
AGROVOC Term:	Agricultural products
AGROVOC Term:	Growth media
AGROVOC Term:	Plant growth stimulants
AGROVOC Term:	Plantations
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10445

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