Identification of bacteriological quality and antimicrobial resistance of microorganisms isolated from animal foods collected from the abattoir butcher shops and local seafood market

Rajani Chowdary A., . and Vijayalakshmi P., . and Vidyullatha P., . and Sharon Sushma M., . Identification of bacteriological quality and antimicrobial resistance of microorganisms isolated from animal foods collected from the abattoir butcher shops and local seafood market. pp. 144-151. ISSN 2550-2166

The current study aimed to isolate bacteria that harbour various animal food products like meat chicken and seafoods collected from the abattoir butcher shops and local seafood market and to determine the antimicrobial resistance pattern of isolated pathogens which are responsible for various foodborne illnesses in human beings. A total of forty raw animal product samples were collected from the abattoir butcher shops and local seafood market of Visakhapatnam. The samples selected for the study include raw chicken meat crab prawns and different varieties of fish. A classic random sampling technique was employed to collect the study samples. All the samples were processed immediately using standard microbiological protocols. The bacteria isolation and characterization were done by studying morphological characteristics with staining methods cultural characteristics by isolating and growing the pathogenic microorganisms in various selective and differential culture media. Antimicrobial susceptibility testing was performed by the Kirby -Bauer method by following Clinical and Laboratory Standards Institute (CLSI) guidelines. EDTA-Disc Potentiation Test and Imipenem-EDTA Double disc synergy test are used to detect the metallo beta-lactamase production of isolated pathogens. The highest number of isolates belong to Salmonella species (18) Pseudomonas aeruginosa (18) followed by Vibrio species (14) and few isolates belong to Enterobacter species (4). Majority of the microbial isolates obtained in the current study were multidrug resistant. The isolates from the abattoir environments slaughterhouses fish markets were found to exhibit variable resistance pattern to aminoglycosides macrolides -lactams cephalosporins quinolone antibiotics used in the present study and at the same time most of them were sensitive to carbapenem antibiotic imipenem. Antimicrobial resistance (AMR) prevents the designing and assessment of effective interventions. If such a link can be established then the tracking of antibiotic use and consumption data could be furthermore used as a surrogate indicator for the risk of potential antibiotic resistance (ABR) emergence.

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Abstract

The current study aimed to isolate bacteria that harbour various animal food products like meat chicken and seafoods collected from the abattoir butcher shops and local seafood market and to determine the antimicrobial resistance pattern of isolated pathogens which are responsible for various foodborne illnesses in human beings. A total of forty raw animal product samples were collected from the abattoir butcher shops and local seafood market of Visakhapatnam. The samples selected for the study include raw chicken meat crab prawns and different varieties of fish. A classic random sampling technique was employed to collect the study samples. All the samples were processed immediately using standard microbiological protocols. The bacteria isolation and characterization were done by studying morphological characteristics with staining methods cultural characteristics by isolating and growing the pathogenic microorganisms in various selective and differential culture media. Antimicrobial susceptibility testing was performed by the Kirby -Bauer method by following Clinical and Laboratory Standards Institute (CLSI) guidelines. EDTA-Disc Potentiation Test and Imipenem-EDTA Double disc synergy test are used to detect the metallo beta-lactamase production of isolated pathogens. The highest number of isolates belong to Salmonella species (18) Pseudomonas aeruginosa (18) followed by Vibrio species (14) and few isolates belong to Enterobacter species (4). Majority of the microbial isolates obtained in the current study were multidrug resistant. The isolates from the abattoir environments slaughterhouses fish markets were found to exhibit variable resistance pattern to aminoglycosides macrolides -lactams cephalosporins quinolone antibiotics used in the present study and at the same time most of them were sensitive to carbapenem antibiotic imipenem. Antimicrobial resistance (AMR) prevents the designing and assessment of effective interventions. If such a link can be established then the tracking of antibiotic use and consumption data could be furthermore used as a surrogate indicator for the risk of potential antibiotic resistance (ABR) emergence.

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Item Type:	Article
AGROVOC Term:	Animal feeding stuffs
AGROVOC Term:	Bacteria
AGROVOC Term:	Culture media
AGROVOC Term:	Pathogenic bacteria
AGROVOC Term:	Identification
AGROVOC Term:	Antibiotic properties
AGROVOC Term:	Bacteriological analysis
AGROVOC Term:	Antimicrobial resistance
AGROVOC Term:	Salmonella
AGROVOC Term:	Antibiotic resistance
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10467

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