Molecular identification of Rusa timorensis (javan rusa) and Rusa unicolor (sambar deer) based on mitochondrial cytochrome c oxidase subunit I (COI)

Fatin Nabilah A., . and Nurain Izzati S., . Molecular identification of Rusa timorensis (javan rusa) and Rusa unicolor (sambar deer) based on mitochondrial cytochrome c oxidase subunit I (COI). pp. 83-85. ISSN 2180-3897

Locally venison is considered as a premium and exotic meat as it is not commonly found in fresh food markets and grocery stores. Despite its limited availability demand is always high in its niche market especially during festive seasons which highly escalate the price. However as an expensive delicacy deer meat is highly susceptible to fraudulent substitution and adulteration. Authentic deer meat are currently only recognized by consumers based on their own experience meat texture and taste which can be quite subjective. To assist in authenticating local deer meat in the market and protect consumers from fraudsters Polymerase Chain Reaction (PCR) analysis can be carried out to distinguish between venison and other animal meat and products. Farmed venison in Malaysia are mostly from the species Rusa timorensis while Rusa unicolor is bred in the wild. Here we detailed a newly developed conventional PCR method that is able to detect R. timorensis and R. unicolor based on partial mitochondrial cytochrome c oxidase subunit I (COI) in a single run thus providing a simple and more accurate alternative in venison authentication.

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Abstract

Locally venison is considered as a premium and exotic meat as it is not commonly found in fresh food markets and grocery stores. Despite its limited availability demand is always high in its niche market especially during festive seasons which highly escalate the price. However as an expensive delicacy deer meat is highly susceptible to fraudulent substitution and adulteration. Authentic deer meat are currently only recognized by consumers based on their own experience meat texture and taste which can be quite subjective. To assist in authenticating local deer meat in the market and protect consumers from fraudsters Polymerase Chain Reaction (PCR) analysis can be carried out to distinguish between venison and other animal meat and products. Farmed venison in Malaysia are mostly from the species Rusa timorensis while Rusa unicolor is bred in the wild. Here we detailed a newly developed conventional PCR method that is able to detect R. timorensis and R. unicolor based on partial mitochondrial cytochrome c oxidase subunit I (COI) in a single run thus providing a simple and more accurate alternative in venison authentication.

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Item Type:	Article
AGROVOC Term:	Deer
AGROVOC Term:	Mitochondrial genetics
AGROVOC Term:	Meat animals
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Determination of species
AGROVOC Term:	Identification
AGROVOC Term:	Meat quality
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10509

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