In vitro propagation of smilax myosotiflora (Ubi Jaga) for conservation and commercial purposes

Rozidah Khalid, . and Nor Hasnida Hassan, . and Nazirah Abdullah, . and Rohani Abdullah, . and Muhammad Fuad Yahya, . and Mohd Saifuldullah Abd Wahid, . and Sabariah Ramuddin, . In vitro propagation of smilax myosotiflora (Ubi Jaga) for conservation and commercial purposes. pp. 110-113. ISSN 2462-1757

In vitro propagation technique has been known to efficiently mass propagate valuable plant species. Threatened species commercial valued and medicinal species are species that suitable to be propagated using tissue culture technology. Smilax sp. a genus belongs to Smilacaceae family has about 350 species and is reported distributed in tropical subtropical and temperate region. Indigenous people from America and Asia have been using the Smilax rhizomes as tonic diuretic and sudorific for century. The morphology of Smilax sp. consists of long thin thorny stem branches with tendrils which attach to other plants or objects and grow steadily upward. Smilax myosotiflora locally known as Ubi jaga in Malaysia has been used in traditional practice as an energy booster and for man health. The rhizome extract was proven to increase men sexual drive and the leaves were reported to have potential to cure syphilis. Currently the source of the raw materials was collected from the wild where difficulty arises during harvesting the rhizome from the soil. Special technique is required to harvest the rhizome and identification of the S. myosotiflora is quite difficult since the morphology between Smilax sp. is very much similar to each other. Due to these issues an alternative for sustainable production of S. myosotiflora raw material is required hence production of this species in tissue culture has been taken and reported in this paper. S. myosotiflora has been introduced into tissue culture using nodal segment. The introduced clean cultures have been propagated in culture media consist of Murashige and Skoog (MS) basal medium supplemented with different range of 6-Benzylaminopurine BAP for shoot multiplication and Indole-3-butyric acid IBA for root induction. The shoot multiplication and root induction medium of S. myosotiflora have been determined where MS basal medium supplemented with 2.0 mg/L BAP induced the highest percentage of shoot formation whereas MS basal medium added with 0.5 mg/L IBA induced the highest percentage of shoot rooted in vitro. The complete plantlets obtained were used for acclimatization experiment in the nursery.

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Abstract

In vitro propagation technique has been known to efficiently mass propagate valuable plant species. Threatened species commercial valued and medicinal species are species that suitable to be propagated using tissue culture technology. Smilax sp. a genus belongs to Smilacaceae family has about 350 species and is reported distributed in tropical subtropical and temperate region. Indigenous people from America and Asia have been using the Smilax rhizomes as tonic diuretic and sudorific for century. The morphology of Smilax sp. consists of long thin thorny stem branches with tendrils which attach to other plants or objects and grow steadily upward. Smilax myosotiflora locally known as Ubi jaga in Malaysia has been used in traditional practice as an energy booster and for man health. The rhizome extract was proven to increase men sexual drive and the leaves were reported to have potential to cure syphilis. Currently the source of the raw materials was collected from the wild where difficulty arises during harvesting the rhizome from the soil. Special technique is required to harvest the rhizome and identification of the S. myosotiflora is quite difficult since the morphology between Smilax sp. is very much similar to each other. Due to these issues an alternative for sustainable production of S. myosotiflora raw material is required hence production of this species in tissue culture has been taken and reported in this paper. S. myosotiflora has been introduced into tissue culture using nodal segment. The introduced clean cultures have been propagated in culture media consist of Murashige and Skoog (MS) basal medium supplemented with different range of 6-Benzylaminopurine BAP for shoot multiplication and Indole-3-butyric acid IBA for root induction. The shoot multiplication and root induction medium of S. myosotiflora have been determined where MS basal medium supplemented with 2.0 mg/L BAP induced the highest percentage of shoot formation whereas MS basal medium added with 0.5 mg/L IBA induced the highest percentage of shoot rooted in vitro. The complete plantlets obtained were used for acclimatization experiment in the nursery.

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Item Type:	Article
AGROVOC Term:	Plant propagation
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Plant cultivation
AGROVOC Term:	Nature conservation
AGROVOC Term:	Sampling
AGROVOC Term:	Data collection
AGROVOC Term:	Researchers
AGROVOC Term:	Viability
AGROVOC Term:	Sustainable development
AGROVOC Term:	Preservation
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10515

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