Molecular characterisation and reporter gene assay of the three GnRH tsoforms and two GnRH receptors of a Clupeiform fish Japanese Sardine Sardinops sagax melanostictus (Temminck Schlegel 1846)

Lumayno Sanny David Pacheco, . and Ohta Kohei, . and Yamaguchi Akihiko, . and Matsuyama Michiya, . Molecular characterisation and reporter gene assay of the three GnRH tsoforms and two GnRH receptors of a Clupeiform fish Japanese Sardine Sardinops sagax melanostictus (Temminck Schlegel 1846). pp. 26-43. ISSN 2073-3720

The gonadotropin-releasing hormone (GnRH) is an important gene involved in fish reproduction and its action is mediated by GnRH receptor (GnRHR). Numerous GnRH-GnRHR studies have already been published in many teleost orders but information on reproduction-related genes for Clupeiformes is limited. To acquire a better understanding on the reproduction of Clupeiform fish species GnRH isoforms and GnRHR paralogues of Japanese sardine Sardinops sagax melanostictus (Temminck Schlegel 1846) were sequenced. Three GnRH isoforms (named as jsGnRH1 jsGnRH2 and jsGnRH3) and two GnRHR paralogues (named as jsGnRHR1 and jsGnRHR2) were isolated in the brain and pituitary of Japanese sardine. Based on phylogenetic analysis jsGnRH1 jsGnRH2 and jsGnRH3 grouped into GnRH1 GnRH2 and GnRH3 forms respectively while jsGnRHR1 and jsGnRHR2 clustered into Type 2b and Type 1c receptors correspondingly. The reporter gene assay showed high binding affinity of jsGnRHR1 to all three GnRH synthetic decapeptides whereas jsGnRHR2 responded best to jsGnRH2 only. Clustering of jsGnRH1and jsGnRHR1 to other teleost GnRH1 and GnRHR2b respectively and activating the calcineurin/NFAT signalling pathway suggests that these two genes are likely involved in fish reproduction.

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Abstract

The gonadotropin-releasing hormone (GnRH) is an important gene involved in fish reproduction and its action is mediated by GnRH receptor (GnRHR). Numerous GnRH-GnRHR studies have already been published in many teleost orders but information on reproduction-related genes for Clupeiformes is limited. To acquire a better understanding on the reproduction of Clupeiform fish species GnRH isoforms and GnRHR paralogues of Japanese sardine Sardinops sagax melanostictus (Temminck Schlegel 1846) were sequenced. Three GnRH isoforms (named as jsGnRH1 jsGnRH2 and jsGnRH3) and two GnRHR paralogues (named as jsGnRHR1 and jsGnRHR2) were isolated in the brain and pituitary of Japanese sardine. Based on phylogenetic analysis jsGnRH1 jsGnRH2 and jsGnRH3 grouped into GnRH1 GnRH2 and GnRH3 forms respectively while jsGnRHR1 and jsGnRHR2 clustered into Type 2b and Type 1c receptors correspondingly. The reporter gene assay showed high binding affinity of jsGnRHR1 to all three GnRH synthetic decapeptides whereas jsGnRHR2 responded best to jsGnRH2 only. Clustering of jsGnRH1and jsGnRHR1 to other teleost GnRH1 and GnRHR2b respectively and activating the calcineurin/NFAT signalling pathway suggests that these two genes are likely involved in fish reproduction.

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Item Type:	Article
AGROVOC Term:	Clupeiformes
AGROVOC Term:	Fishes
AGROVOC Term:	Sardinops sagax
AGROVOC Term:	Gonadotropin releasing hormone
AGROVOC Term:	Gene sequence
AGROVOC Term:	Gene expression
AGROVOC Term:	Reproduction
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10605

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