Correlation of the GC-MS-based metabolite profile of Momordica charantia fruit and its antioxidant activity

F. Abas, . and El-Seedi H., . and Khatib A., . and V. Perumal, . and M. Z. Saiman, . and Ahmed Q. U., . and Uzir B. F., . and S. Murugesu, . and R. Primaharinastiti, . Correlation of the GC-MS-based metabolite profile of Momordica charantia fruit and its antioxidant activity. pp. 58-66. ISSN 22317546

Momordica charantia or bitter melon (Cucurbitaceae) is a widely consumed edible fruit with strong antioxidant properties. Due to these properties it has been commercialised by the natural product industries as a coadjutant in the treatment of various ailments attributable to the deleterious effects of oxidants. The present work aimed to evaluate the antioxidant activity of M. charantia fruit extracts made with different compositions of ethanol:water and to identify the metabolites that are responsible for this activity. To this end the fruit samples were extracted using six different concentrations of ethanol in water (0 20 40 60 80 and 100). Gas chromatography-mass spectrometry (GC-MS) and multivariate data analysis (MVDA) were used to identify significant antioxidants. The 80 ethanol:water extract showed the most significant (p 0.05) antioxidant activity when tested with the 1 1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) antioxidant assays. The multivariate data analysis revealed that the metabolites related to this antioxidant activity were gentiobiose glucose galactonic acid palmitic acid galactose mannose and fructose.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/29%20(01)%202022/DONE%2...

Abstract

Momordica charantia or bitter melon (Cucurbitaceae) is a widely consumed edible fruit with strong antioxidant properties. Due to these properties it has been commercialised by the natural product industries as a coadjutant in the treatment of various ailments attributable to the deleterious effects of oxidants. The present work aimed to evaluate the antioxidant activity of M. charantia fruit extracts made with different compositions of ethanol:water and to identify the metabolites that are responsible for this activity. To this end the fruit samples were extracted using six different concentrations of ethanol in water (0 20 40 60 80 and 100). Gas chromatography-mass spectrometry (GC-MS) and multivariate data analysis (MVDA) were used to identify significant antioxidants. The 80 ethanol:water extract showed the most significant (p 0.05) antioxidant activity when tested with the 1 1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) antioxidant assays. The multivariate data analysis revealed that the metabolites related to this antioxidant activity were gentiobiose glucose galactonic acid palmitic acid galactose mannose and fructose.

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Item Type:	Article
AGROVOC Term:	Momordica charantia
AGROVOC Term:	Cucurbitaceae
AGROVOC Term:	Fruit extracts
AGROVOC Term:	Metabolites
AGROVOC Term:	Ethanol
AGROVOC Term:	Gas chromatography
AGROVOC Term:	Mass spectrometry
AGROVOC Term:	Multivariate analysis
AGROVOC Term:	Gentiobiose
AGROVOC Term:	Galactose
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10617

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