Aflatoxin M‚� reduction by microorganisms isolated from kefir grains


Citation

Rahayu W. P., . and Adriansyah P. N. A., . and Kusumaningrum H. D., . and Kawamura O., . Aflatoxin M‚� reduction by microorganisms isolated from kefir grains. pp. 78-85. ISSN 22317546

Abstract

Aflatoxin M‚�(AFM‚�) is a mycotoxin that often contaminates milk. Like other mycotoxins it is thermostable and potentially carcinogenic. The present work was carried out to evaluate the ability of microorganisms isolated from Indonesian kefir grains to reduce AFM‚� in contaminated phosphate buffer saline (PBS). Fourteen isolates of lactic acid bacteria both aerobic (LAE) and anaerobic (LAN) and nine isolates of yeast (YEA) were used. The significantly highest AFM‚� reduction percentage was shown by the isolate LAE7 (29.3 0.6) after 4 h incubation. DNA sequencing of LAE7 and YEA2 isolates showed that these isolates had homology (level of similarity) with species of Lactobacillus kefiri strain A/K and Saccharomyces cerevisiae NRRL Y-12632 respectively. The present work proved that isolates from Indonesian kefir grains could reduce AFM‚� and have the potential for practical use.


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Abstract

Aflatoxin M‚�(AFM‚�) is a mycotoxin that often contaminates milk. Like other mycotoxins it is thermostable and potentially carcinogenic. The present work was carried out to evaluate the ability of microorganisms isolated from Indonesian kefir grains to reduce AFM‚� in contaminated phosphate buffer saline (PBS). Fourteen isolates of lactic acid bacteria both aerobic (LAE) and anaerobic (LAN) and nine isolates of yeast (YEA) were used. The significantly highest AFM‚� reduction percentage was shown by the isolate LAE7 (29.3 0.6) after 4 h incubation. DNA sequencing of LAE7 and YEA2 isolates showed that these isolates had homology (level of similarity) with species of Lactobacillus kefiri strain A/K and Saccharomyces cerevisiae NRRL Y-12632 respectively. The present work proved that isolates from Indonesian kefir grains could reduce AFM‚� and have the potential for practical use.

Additional Metadata

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Item Type: Article
AGROVOC Term: Aflatoxins
AGROVOC Term: Kefir
AGROVOC Term: Mycotoxins
AGROVOC Term: Reduction
AGROVOC Term: Isolation of microorganisms
AGROVOC Term: Lactic acid bacteria
AGROVOC Term: Fungal contamination
AGROVOC Term: Yeasts
AGROVOC Term: best practices
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:55
URI: http://webagris.upm.edu.my/id/eprint/10619

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