Multi-strain probiotics combined with fruit-vegetable powders for regulating intestinal inflammation and intestinal epithelial barrier

Lin Y. H., . and Hsu Y. C., . and Lin P., . and Lin Y. K., . and Lin Y. H., . and Chiang C. F., . Multi-strain probiotics combined with fruit-vegetable powders for regulating intestinal inflammation and intestinal epithelial barrier. pp. 258-264. ISSN 22317546

The health-promoting effects of probiotics include maintenance of normal intestinal microbiota increased nutritional value of foods and immune system stimulation. Multi-strain probiotics have recently been proposed as health-enhancing foods and functional food ingredients. Fruit-vegetable powders (FVP) being a kind of prebiotic are food supplements that are non-digestible by the host but can improve the host's health by selectively stimulating the growth or activities of gastrointestinal tract bacteria. However the intestinal efficacy of multi-strain probiotics combined with FVP remains unclear. Therefore the purpose of the present work was to explore the effect of multi-strain probiotics combined with FVP on intestinal inflammation. Lipopolysaccharide (LPS) was used to treat RAW264.7 which was then co-cultured with Caco-2 cells to mimic the intestinal inflammatory environment. Caco2 cells were incubated with various probiotics and FVP (0.125 and 0.25 mg/mL). The inflammatory cytokines from the medium were collected for ELISA analysis and the ZO-1 expression in the Caco-2 cells was examined by fluorescence assay. Probiotics combined with FVP significantly decreased the inflammatory cytokines IL-6 and TNF- and increased ZO1 expression when compared with the LPS only group. Probiotics combined with FVP could decrease inflammatory cytokines and protect the intestinal barrier from tight junction dysregulation

Full text available from: Official URL: http://www.ifrj.upm.edu.my/29%20(02)%202022/04%20-...

Abstract

The health-promoting effects of probiotics include maintenance of normal intestinal microbiota increased nutritional value of foods and immune system stimulation. Multi-strain probiotics have recently been proposed as health-enhancing foods and functional food ingredients. Fruit-vegetable powders (FVP) being a kind of prebiotic are food supplements that are non-digestible by the host but can improve the host's health by selectively stimulating the growth or activities of gastrointestinal tract bacteria. However the intestinal efficacy of multi-strain probiotics combined with FVP remains unclear. Therefore the purpose of the present work was to explore the effect of multi-strain probiotics combined with FVP on intestinal inflammation. Lipopolysaccharide (LPS) was used to treat RAW264.7 which was then co-cultured with Caco-2 cells to mimic the intestinal inflammatory environment. Caco2 cells were incubated with various probiotics and FVP (0.125 and 0.25 mg/mL). The inflammatory cytokines from the medium were collected for ELISA analysis and the ZO-1 expression in the Caco-2 cells was examined by fluorescence assay. Probiotics combined with FVP significantly decreased the inflammatory cytokines IL-6 and TNF- and increased ZO1 expression when compared with the LPS only group. Probiotics combined with FVP could decrease inflammatory cytokines and protect the intestinal barrier from tight junction dysregulation

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Item Type:	Article
AGROVOC Term:	Probiotics
AGROVOC Term:	Fruit vegetables
AGROVOC Term:	Powders
AGROVOC Term:	Lipopolysaccharides
AGROVOC Term:	Intestinal diseases
AGROVOC Term:	Inflammation
AGROVOC Term:	Food supplements
AGROVOC Term:	Supplements
AGROVOC Term:	Disease treatment
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10634

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