The identification of Chromobacterium violaceum from soil based on the production of violacein

Halimi Mohd Saud, . and Wai Keong Loke, . The identification of Chromobacterium violaceum from soil based on the production of violacein. pp. 33-39. ISSN 2180-1983

Chromobacterium violaceumis a purple coloured pathogenic bacterium mainly found in stagnant water and soil. Isolation and identification of this pathogenic bacteria are very important especially in biological science research and clinical field. It was easily identified by the production of purple coloured pigment violacein based on the solubility colour reaction spectrophotometry and growth temperature of C. violaceum. The density of C. violaceum found in 5 g of soil was much higher after rainy day which was about 7 X 10�· located at 259'09.5N 10143'33.2E and 1 X 10�· located at 258'56.3N 10143'15.2E compared to normal sunny day with only 6 X 10� and 9 X 10�´ in respectively at the same locations. The soils samples were collected 0-5 cm depth from the soil surface because of the facultative anaerobic where the C. violaceum from underground was more incline go to the top of soil since the concentration of oxygen was higher at the top.

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Abstract

Chromobacterium violaceumis a purple coloured pathogenic bacterium mainly found in stagnant water and soil. Isolation and identification of this pathogenic bacteria are very important especially in biological science research and clinical field. It was easily identified by the production of purple coloured pigment violacein based on the solubility colour reaction spectrophotometry and growth temperature of C. violaceum. The density of C. violaceum found in 5 g of soil was much higher after rainy day which was about 7 X 10�· located at 259'09.5N 10143'33.2E and 1 X 10�· located at 258'56.3N 10143'15.2E compared to normal sunny day with only 6 X 10� and 9 X 10�´ in respectively at the same locations. The soils samples were collected 0-5 cm depth from the soil surface because of the facultative anaerobic where the C. violaceum from underground was more incline go to the top of soil since the concentration of oxygen was higher at the top.

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Item Type:	Article
AGROVOC Term:	Bacteria
AGROVOC Term:	Gram negative bacteria
AGROVOC Term:	Soil
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Identification
AGROVOC Term:	Pathogenic bacteria
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10656

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