Effects of interesterified fats on lipoprotein sub fractions and hepatic gene expressions in a hamster model

Kanga Rani Selvaduray, . and Goh Yong Meng, . and Gowri Nagapan, . and Noor Lida Habi Mat Dian, . and Che Anishas Che Idris, . and Nagendran Balasundram, . Effects of interesterified fats on lipoprotein sub fractions and hepatic gene expressions in a hamster model. pp. 356-367. ISSN 2811-4701

Palmitic rich interesterified (IE) fats exert detrimental effects on atherogenicity in animals but less significant effects in animal and human plasma lipids. Thus it is important to investigate the role IE fats on lipid subfractions and hepatic gene expression involved in lipoprotein regulation. F1B male Golden Syrian hamsters (Mesocricetus auratus) were fed high-fat diets ad libitum containing 0.1 dietary cholesterol and 30.0 energy from dietary fat either native or IE fats namely palm olein (PO) chemically IE palm olein (CIEPO) sal fat blend (SFB) and chemically IE sal fat blend (CIESFB) for 12 weeks. Plasma lipid profiles low density lipoprotein (LDL) and high density lipoprotein (HDL) sub-fractions and hepatic gene expression levels were determined. PO and CIEPO fed hamsters had 38 and 27 higher plasma HDL levels compared to SFB and CIESFB respectively. Animals given PO diet had greater proportion of the larger HDL particles than SFB and CIESFB fed animals (p0.05). Whereas animals fed with SFB and CIESFB had greater proportion of larger LDL particles compared to both palmitic counterparts. All diets upregulated genes involved in liver fat accumulation such as CXCL16 VLDR and APO E. SFB diet showed significant (p0.05) 16-fold upregulation in CXCL16 gene. Gene expression for ABCA1 APO A1 and CETP were upregulated all groups in response of reverse cholesterol transport (RCT). Palmitic-rich diets presented significant upregulation in APO A1 gene (p0.05). LDL metabolism related genes such as LDLR PCSK9 APO B CYP7A1 PCSK9 were downregulated in all diets. In conclusion native and IE saturated high-fat diets induce liver steatosis in hamsters as shown in CXCL16 VLDLR and APOE expression. In this condition cholesterol clearance via RCT was activated with expression of related genes such as ABCA1 LCAT APO A1 and CETP. However these effects on plasma level HDL cholesterol and large HDL sub-fractions were only seen in palmitic rich fats. Whereas LDLR mediated cholesterol clearance was downregulated with suppression of LDLR gene with similar effects on plasma LDL in all diets.

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Abstract

Palmitic rich interesterified (IE) fats exert detrimental effects on atherogenicity in animals but less significant effects in animal and human plasma lipids. Thus it is important to investigate the role IE fats on lipid subfractions and hepatic gene expression involved in lipoprotein regulation. F1B male Golden Syrian hamsters (Mesocricetus auratus) were fed high-fat diets ad libitum containing 0.1 dietary cholesterol and 30.0 energy from dietary fat either native or IE fats namely palm olein (PO) chemically IE palm olein (CIEPO) sal fat blend (SFB) and chemically IE sal fat blend (CIESFB) for 12 weeks. Plasma lipid profiles low density lipoprotein (LDL) and high density lipoprotein (HDL) sub-fractions and hepatic gene expression levels were determined. PO and CIEPO fed hamsters had 38 and 27 higher plasma HDL levels compared to SFB and CIESFB respectively. Animals given PO diet had greater proportion of the larger HDL particles than SFB and CIESFB fed animals (p0.05). Whereas animals fed with SFB and CIESFB had greater proportion of larger LDL particles compared to both palmitic counterparts. All diets upregulated genes involved in liver fat accumulation such as CXCL16 VLDR and APO E. SFB diet showed significant (p0.05) 16-fold upregulation in CXCL16 gene. Gene expression for ABCA1 APO A1 and CETP were upregulated all groups in response of reverse cholesterol transport (RCT). Palmitic-rich diets presented significant upregulation in APO A1 gene (p0.05). LDL metabolism related genes such as LDLR PCSK9 APO B CYP7A1 PCSK9 were downregulated in all diets. In conclusion native and IE saturated high-fat diets induce liver steatosis in hamsters as shown in CXCL16 VLDLR and APOE expression. In this condition cholesterol clearance via RCT was activated with expression of related genes such as ABCA1 LCAT APO A1 and CETP. However these effects on plasma level HDL cholesterol and large HDL sub-fractions were only seen in palmitic rich fats. Whereas LDLR mediated cholesterol clearance was downregulated with suppression of LDLR gene with similar effects on plasma LDL in all diets.

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Item Type:	Article
AGROVOC Term:	Palm oils
AGROVOC Term:	Lipoproteins
AGROVOC Term:	Dietary components
AGROVOC Term:	Experimental animals
AGROVOC Term:	Hamsters
AGROVOC Term:	Fatty acids
AGROVOC Term:	Gene expression
AGROVOC Term:	Body weight
AGROVOC Term:	Fat content
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10684

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