Simplified approach for early identification of spontaneous oil palm haploid (Elaeis guineensis)

Muhammad Azwan Z., . and Zaki N. M., . and Nordiana H. M. N., . and Madon M., . and Norziha A., . and Zulkifli Y., . and Chan P. L., . and Singh R., . Simplified approach for early identification of spontaneous oil palm haploid (Elaeis guineensis). pp. 394-400. ISSN 2811-4701

Haploid technology facilitates the production of completely homozygous plants that are desirable in crop breeding. Having just one complete set of chromosomes in a haploid individual allows it to be doubled to produce a normal but pure 2n diploid plant. Here we report a simple way to identify natural haploids of oil palm (Elaeis guineensis Jacq.) from screening 6400 abnormal germinated seeds. Initially the germinated seeds were selected based on 12 unique off-type morphological characteristics. The selected seeds were then grown and the seedlings were subjected to a second selection for three distinctive characteristics. Ploidy analysis with flow cytometry (FCM) and chromosome karyotyping confirmed the haploidy of one seedling with stunted height and size. Further analysis with the True-to-Type single nucleotide polymorphism (SNP) panel demonstrated that the plant was homozygous at all the loci tested confirming its haploid status. This study has established a simple and systematic strategy that assists in accelerating early identification of oil palm spontaneous haploid.

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Abstract

Haploid technology facilitates the production of completely homozygous plants that are desirable in crop breeding. Having just one complete set of chromosomes in a haploid individual allows it to be doubled to produce a normal but pure 2n diploid plant. Here we report a simple way to identify natural haploids of oil palm (Elaeis guineensis Jacq.) from screening 6400 abnormal germinated seeds. Initially the germinated seeds were selected based on 12 unique off-type morphological characteristics. The selected seeds were then grown and the seedlings were subjected to a second selection for three distinctive characteristics. Ploidy analysis with flow cytometry (FCM) and chromosome karyotyping confirmed the haploidy of one seedling with stunted height and size. Further analysis with the True-to-Type single nucleotide polymorphism (SNP) panel demonstrated that the plant was homozygous at all the loci tested confirming its haploid status. This study has established a simple and systematic strategy that assists in accelerating early identification of oil palm spontaneous haploid.

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Item Type:	Article
AGROVOC Term:	Oil palm
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Haploids
AGROVOC Term:	Seeds
AGROVOC Term:	Seed germination
AGROVOC Term:	Identification
AGROVOC Term:	Flow cytometry (cells)
AGROVOC Term:	Detectors
AGROVOC Term:	Plant breeding
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10687

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