Perlite-immobilized bacterial consortium enhanced degradation of crude oil-contaminated marine sediment

Uyun Kurrotul, . and Darmayati Yeti, . and Irfan Mustafa, . Perlite-immobilized bacterial consortium enhanced degradation of crude oil-contaminated marine sediment. pp. 194-203. ISSN 2672-7226

The limit biodegradation of oil pollutant is their limited availability to microorganisms because application in the field uses liquid bacterial culture which makes the resistance and ability of bacteria become susceptible to being disturbed due to an inappropriate environment. Immobilization of cells is one way to overcome this problem and selecting suitable materials to protect bacteria is an important thing. The study aims to compare the crude oil biodegradation ability of bacterial consortium consisting of Enterobacter cloacae Bacillus licheniformis and Bacillus sp. in the form of free and immobilized cells using microcosms design. In this study immobilized was used to immobilized bacteria on perlite as an inorganic carrier. Bacterial cells trapped in the perlite were counted to determine the entrapment capacity. The efficiency of biodegradation was evaluated every week for 28 days by measuring total petroleum hydrocarbon and another variable such as total number of bacteria and environmental changes. The results of crude oil biodegradation efficiency showed there was not significantly between immobilized cells (63.86 2.29) that more efficient than the free cells (38.55 0.34) after 28 days of incubation. Based on our result perlite can be use suitable carrier to immobilized cell.

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Abstract

The limit biodegradation of oil pollutant is their limited availability to microorganisms because application in the field uses liquid bacterial culture which makes the resistance and ability of bacteria become susceptible to being disturbed due to an inappropriate environment. Immobilization of cells is one way to overcome this problem and selecting suitable materials to protect bacteria is an important thing. The study aims to compare the crude oil biodegradation ability of bacterial consortium consisting of Enterobacter cloacae Bacillus licheniformis and Bacillus sp. in the form of free and immobilized cells using microcosms design. In this study immobilized was used to immobilized bacteria on perlite as an inorganic carrier. Bacterial cells trapped in the perlite were counted to determine the entrapment capacity. The efficiency of biodegradation was evaluated every week for 28 days by measuring total petroleum hydrocarbon and another variable such as total number of bacteria and environmental changes. The results of crude oil biodegradation efficiency showed there was not significantly between immobilized cells (63.86 2.29) that more efficient than the free cells (38.55 0.34) after 28 days of incubation. Based on our result perlite can be use suitable carrier to immobilized cell.

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Item Type:	Article
AGROVOC Term:	Crude oil
AGROVOC Term:	Perlite
AGROVOC Term:	Immobilized bacteria
AGROVOC Term:	Enterobacter cloacae
AGROVOC Term:	Bacillus licheniformis
AGROVOC Term:	Bacillus sphaericus
AGROVOC Term:	Immobilized bacteria
AGROVOC Term:	Inorganic compounds
AGROVOC Term:	Biodegradation
AGROVOC Term:	Bioremediation
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10694

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