Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors

Faez Firdaus Abdullah Jesse, . and Mohamad Fakhri Yaacob, . and Mohd Fakharul Zaman Raja Yahya, . and Syaida Anati Abd Rashid, . and Mohd Shafiq Aazmi, . Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors. pp. 49-56. ISSN 2672-7226

Corynebacterium pseudotuberculosis represents the causative factor of caseous lymphadenitis a ruminant disease that contributes to major economic loss in most sheep farming countries. The present study was performed to determine the efficacy of DNA synthesis (nalidixic acid) and protein synthesis inhibitors (streptomycin and tetracycline) on C. pseudotuberculosis biofilm. Biofilm of C. pseudotuberculosis clinical isolate was developed in the flat-bottom microplate. Field-emission scanning electron microscope and resazurin assay were used to investigate the structure and antimicrobial susceptibility of C. pseudotuberculosis biofilm respectively. All test inhibitors were evaluated in the range between 3.125 g/ml and 100 g/ml. Results demonstrated that C. pseudotuberculosis biofilm formed a three dimensional and heterogeneous structure on the surface. The highest biofilm percentage inhibition shown by nalidixic acid streptomycin and tetracycline were 71.57 87.44 and 74.73 respectively. Significant (p0.05) correlation of antibiofilm susceptibility between these inhibitors and other antimicrobials were also demonstrated herein. The findings of the present study suggest the potential use of streptomycin for the control of caseous lymphadenitis.

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Abstract

Corynebacterium pseudotuberculosis represents the causative factor of caseous lymphadenitis a ruminant disease that contributes to major economic loss in most sheep farming countries. The present study was performed to determine the efficacy of DNA synthesis (nalidixic acid) and protein synthesis inhibitors (streptomycin and tetracycline) on C. pseudotuberculosis biofilm. Biofilm of C. pseudotuberculosis clinical isolate was developed in the flat-bottom microplate. Field-emission scanning electron microscope and resazurin assay were used to investigate the structure and antimicrobial susceptibility of C. pseudotuberculosis biofilm respectively. All test inhibitors were evaluated in the range between 3.125 g/ml and 100 g/ml. Results demonstrated that C. pseudotuberculosis biofilm formed a three dimensional and heterogeneous structure on the surface. The highest biofilm percentage inhibition shown by nalidixic acid streptomycin and tetracycline were 71.57 87.44 and 74.73 respectively. Significant (p0.05) correlation of antibiofilm susceptibility between these inhibitors and other antimicrobials were also demonstrated herein. The findings of the present study suggest the potential use of streptomycin for the control of caseous lymphadenitis.

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Item Type:	Article
AGROVOC Term:	Corynebacterium pseudotuberculosis
AGROVOC Term:	Biofilms (microbial contaminants)
AGROVOC Term:	Protein synthesis
AGROVOC Term:	Tetracyclines
AGROVOC Term:	Streptomycin
AGROVOC Term:	Caseous lymphadenitis
AGROVOC Term:	Growth inhibitors
AGROVOC Term:	Antimicrobials
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10734

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