Histopathological evaluation of rats induced with periodontitis using Enterococcus faecalis and wire ligation of the teeth

Badiah Baharin, . and Farinawati Yazid, . and Nur Najmi Mohamad Anuar, . and Nurrul Shaqinah Nasruddin, . and Fazle Khuda, . Histopathological evaluation of rats induced with periodontitis using Enterococcus faecalis and wire ligation of the teeth. pp. 117-126. ISSN 2672-7226

Gingivitis and periodontitis are periodontal diseases that present as an inflammatory periodontium. Enterococcus faecalis is a gram-positive facultative microorganism that can increase periodontal destruction. High levels of medically important pathogens in the periodontitis associated microbiota may pose a risk to the development of infections at distant body sites. This study aims to investigate histological changes within the periodontium heart and kidney following concurrent infection with ligature wire and E. faecalis in rats. Rats were divided into three groups 0-day (control) 7-day (experimental) and 14-day (experimental) containing four rats each. Sterile 0.2 mm wires were inserted into the inter dental space of the maxillary right first and second molar. A total of 0.5 microliter of E. faecalis suspension (1.5 x 10� CFU/ml) was injected into the gingival sulcus of the same site once a week for two weeks. Following euthanasia maxillae heart and kidney tissue samples were collected processed for hematoxylin and eosin staining accordingly. The results showed hyperplastic gingival epithelium attachment loss and inflammatory cell infiltration within the connective tissue after 7-day. However inflammatory cell infiltration reduced after 14-day. Inflammatory cell infiltration within the myocardium of the heart tissue and congested blood vessels within the glomerulus and inflammatory cell infiltration into the interstitial space were observed in the kidney sample. The findings suggest acute inflammation within the periodontal tissue and developing infection at distant body sites.

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Abstract

Gingivitis and periodontitis are periodontal diseases that present as an inflammatory periodontium. Enterococcus faecalis is a gram-positive facultative microorganism that can increase periodontal destruction. High levels of medically important pathogens in the periodontitis associated microbiota may pose a risk to the development of infections at distant body sites. This study aims to investigate histological changes within the periodontium heart and kidney following concurrent infection with ligature wire and E. faecalis in rats. Rats were divided into three groups 0-day (control) 7-day (experimental) and 14-day (experimental) containing four rats each. Sterile 0.2 mm wires were inserted into the inter dental space of the maxillary right first and second molar. A total of 0.5 microliter of E. faecalis suspension (1.5 x 10� CFU/ml) was injected into the gingival sulcus of the same site once a week for two weeks. Following euthanasia maxillae heart and kidney tissue samples were collected processed for hematoxylin and eosin staining accordingly. The results showed hyperplastic gingival epithelium attachment loss and inflammatory cell infiltration within the connective tissue after 7-day. However inflammatory cell infiltration reduced after 14-day. Inflammatory cell infiltration within the myocardium of the heart tissue and congested blood vessels within the glomerulus and inflammatory cell infiltration into the interstitial space were observed in the kidney sample. The findings suggest acute inflammation within the periodontal tissue and developing infection at distant body sites.

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Item Type:	Article
AGROVOC Term:	Enterococcus faecalis
AGROVOC Term:	Ligature
AGROVOC Term:	Periodontal disease
AGROVOC Term:	Gingivitis
AGROVOC Term:	Histopathology
AGROVOC Term:	Gram positive bacteria
AGROVOC Term:	Inflammation
AGROVOC Term:	Infection
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10739

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