Water quality assessment and the prevalence of antibioticresistant bacteria from a recreational river in Kuching Sarawak Malaysia

Khairunnisa Mohammad Hamdi, . and Lihan Samuel, . and Nurashikin Hamdan, . and Tay Meng Guan, . Water quality assessment and the prevalence of antibioticresistant bacteria from a recreational river in Kuching Sarawak Malaysia. pp. 37-59. ISSN 2672-7226

Recreational activities may affect a rivers water quality which may pose health risks to those in direct contact with the water. This study aims to analyse the water quality if Jangoi River in Kuching Sarawak by measuring the conventional chemical and biological parameters and to characterise the bacteria isolated from the water based on their antibiotic susceptibility. The determination of the water quality is based on the Department of Environments Malaysian Water Quality Index (WQI) which was carried out at three sampling stations (upstream middle stream and downstream) in two different sampling trips. Six water quality parameters were measured: Dissolved oxygen biological oxygen demand pH chemical oxygen demand (COD) ammoniacal nitrogen and total suspended solid. The WQI values of the river ranged from 88 to 92 classifying the river under Class I. The one-way ANOVA analysis revealed that the parameter values for all the three stations are not significantly different except for the COD. The higher COD value of the upstream water could be due to the release of wastewater from houses and agricultural activities near Jangoi River. The faecal coliform and total coliform counts ranged from 650 CFU/100 mL to 1 000 CFU/100 mL and 20 050 CFU/100 mL to 23 250 CFU/100 mL respectively. Nine bacteria were isolated and 16S rRNA PCR and DNA sequencing. The DNA sequencing revealed the presence of Escherichia coli Chromobactium violaceum Lelliota amnigena Pseudomonas aeruginosa Achromobacter xylosoxidans Stenotrophomonas maltophilia Pseudomonas hibiscicola Achromobacter mucicolens and Bacillus pacificus. Antibiotic susceptibility tests demonstrated the highest percentage of susceptibility for ciprofloxacin (100) followed by ampicillin (40) and chloramphenicol (40). However the highest percentage of resistance (60) was shown by erythromycin. The multiple antibiotic resistant (MAR) index in this study ranged from 0.0 to 0.6. The rivers WQI categorisation and microbiological status are inconsistent indicating the need to modify the WQI formula to include the microbial parameter. Additionally this study recommends the establishment of water quality and antibiotic resistance pattern monitoring programmes to anticipate the emergence of MAR bacteria in the aquatic environment and to document the continuous water quality state of Sarawaks recreational rivers.

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Abstract

Recreational activities may affect a rivers water quality which may pose health risks to those in direct contact with the water. This study aims to analyse the water quality if Jangoi River in Kuching Sarawak by measuring the conventional chemical and biological parameters and to characterise the bacteria isolated from the water based on their antibiotic susceptibility. The determination of the water quality is based on the Department of Environments Malaysian Water Quality Index (WQI) which was carried out at three sampling stations (upstream middle stream and downstream) in two different sampling trips. Six water quality parameters were measured: Dissolved oxygen biological oxygen demand pH chemical oxygen demand (COD) ammoniacal nitrogen and total suspended solid. The WQI values of the river ranged from 88 to 92 classifying the river under Class I. The one-way ANOVA analysis revealed that the parameter values for all the three stations are not significantly different except for the COD. The higher COD value of the upstream water could be due to the release of wastewater from houses and agricultural activities near Jangoi River. The faecal coliform and total coliform counts ranged from 650 CFU/100 mL to 1 000 CFU/100 mL and 20 050 CFU/100 mL to 23 250 CFU/100 mL respectively. Nine bacteria were isolated and 16S rRNA PCR and DNA sequencing. The DNA sequencing revealed the presence of Escherichia coli Chromobactium violaceum Lelliota amnigena Pseudomonas aeruginosa Achromobacter xylosoxidans Stenotrophomonas maltophilia Pseudomonas hibiscicola Achromobacter mucicolens and Bacillus pacificus. Antibiotic susceptibility tests demonstrated the highest percentage of susceptibility for ciprofloxacin (100) followed by ampicillin (40) and chloramphenicol (40). However the highest percentage of resistance (60) was shown by erythromycin. The multiple antibiotic resistant (MAR) index in this study ranged from 0.0 to 0.6. The rivers WQI categorisation and microbiological status are inconsistent indicating the need to modify the WQI formula to include the microbial parameter. Additionally this study recommends the establishment of water quality and antibiotic resistance pattern monitoring programmes to anticipate the emergence of MAR bacteria in the aquatic environment and to document the continuous water quality state of Sarawaks recreational rivers.

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Item Type:	Article
AGROVOC Term:	Water quality
AGROVOC Term:	Rivers
AGROVOC Term:	Recreational areas
AGROVOC Term:	Environmental assessment
AGROVOC Term:	Bacteria
AGROVOC Term:	Disease prevalence
AGROVOC Term:	DNA sequence
AGROVOC Term:	Antibiotic resistance
AGROVOC Term:	Environmental monitoring
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10750

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