Application of mild-subcritical alkaline water extraction: optimum condition for phenolic antioxidant extracted from cold-pressed defatted KDML 105 rice bran and its phenolic profile

Sripui J., . and Punnongwa W., . and Sae-Eaw A., . and Thawornchinsombut S., . Application of mild-subcritical alkaline water extraction: optimum condition for phenolic antioxidant extracted from cold-pressed defatted KDML 105 rice bran and its phenolic profile. pp. 120-131. ISSN 2550-2166

This research was aimed at optimizing the process of the mild-subcritical alkaline water (pH 9.5) extraction (mild SAW) of cold-pressed defatted Khao Dawk Mali 105 rice bran (CDRB) in order to achieve the maximum antioxidants with minimal brown colour. Response surface methodology and central composite design were performed with two variables: extraction temperature (107-129C) and time (21-149 mins). The elucidated optimum conditions were 119C and 85 mins with predicted response values: the total phenolic content of 16.9 mg gallic acid/g; protein of 64.3 mg/g; an ABTS radical scavenging activity of 14.4 mg Trolox/g; a browning index of 0.41; and reducing sugar of 62.0 mg/g. Proto-catechuic acid and p-coumaric acid were major phenolic compounds in the CDRB while trans-ferulic acid proto-catechuic acid and p-coumaric acid were all abundant in the mild SAW extract. The results confirmed that the mild SAW extraction process for antioxidants is an environmentally friendly approach which can increase the release of value-added compounds from rice bran residues.

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Abstract

This research was aimed at optimizing the process of the mild-subcritical alkaline water (pH 9.5) extraction (mild SAW) of cold-pressed defatted Khao Dawk Mali 105 rice bran (CDRB) in order to achieve the maximum antioxidants with minimal brown colour. Response surface methodology and central composite design were performed with two variables: extraction temperature (107-129C) and time (21-149 mins). The elucidated optimum conditions were 119C and 85 mins with predicted response values: the total phenolic content of 16.9 mg gallic acid/g; protein of 64.3 mg/g; an ABTS radical scavenging activity of 14.4 mg Trolox/g; a browning index of 0.41; and reducing sugar of 62.0 mg/g. Proto-catechuic acid and p-coumaric acid were major phenolic compounds in the CDRB while trans-ferulic acid proto-catechuic acid and p-coumaric acid were all abundant in the mild SAW extract. The results confirmed that the mild SAW extraction process for antioxidants is an environmentally friendly approach which can increase the release of value-added compounds from rice bran residues.

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Item Type:	Article
AGROVOC Term:	Phenolic content
AGROVOC Term:	Antioxidants
AGROVOC Term:	Rice
AGROVOC Term:	Experiments
AGROVOC Term:	Sampling
AGROVOC Term:	Proximate composition
AGROVOC Term:	Analysis of variance
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Phenolic compounds
AGROVOC Term:	Impact assessment
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10771

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