Physicochemical and protein characterization of lima bean (Phaseolus lunatus L) seed

Estiasih T., . and Palupi H. T., . and Yunianta, . and Sutrisno A., . Physicochemical and protein characterization of lima bean (Phaseolus lunatus L) seed. pp. 168-177. ISSN 2550-2166

Lima bean (Phaseolus lunatus L) is native to Latin America and has spread out worldwide thus having a high level of diversity. The lima bean seed from a specific region might have different characteristics from others. This study was aimed to characterize the physicochemical and protein of the dry seed of lima bean from Indonesia based on its solubility electrophoretic pattern and amino acid profile. The result showed that carbohydrate (68.891.55) was the major component with starch (41.961.10) as the predominant. The amylopectin was higher than amylose. Dietary fibre (27.870.37) was significant and dominated by insoluble one (25.470.32). The fat content (1.150.04) was low and ash (3.670.47) comprised of magnesium phosphorus potassium calcium and iron with the content of 184 75 38 11 and 10 mg/100 g respectively. Total phenolic compounds of this seed were 1.290.02 mg/g phytic acid 11.570.03 mg/g saponins 16.840.42 mg/g and trypsin inhibitors 36.070.11 TIU/g. HCN was found significantly at 30.990.29 ppm. Oligosaccharides were 5.930.29 that comprised of raffinose 1.220.08 and stachyose 4.610.21. Protein content was moderate (15.930.55) that comprised of albumin 18.470.62 globulin 56.202.00 prolamin 3.140.20 and glutelin 22.691.60. The molecular weight of this protein was 10-141 kDa with 12 polypeptides. Globulin had 12 polypeptides of 10 to 124 kDa. Albumin had 18 to 116 kDa molecular weights while glutelin had six polypeptides of 13 to 109 kDa. Prolamin did not have visible polypeptides. Lysine leucine valine and phenylalanine were the primary essential amino acids with high lysine but low methionine and cysteine. In conclusion lima bean seed is a source of carbohydrates and minerals with a moderate protein content dominated by the globulin. The polypeptide profile of lima bean seed is varied depending on the protein fractions. The occurrence of antinutrition might hinder its utilization as a protein source. HCN as a toxicant should be removed to obtain a safe seed for consumption.

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Abstract

Lima bean (Phaseolus lunatus L) is native to Latin America and has spread out worldwide thus having a high level of diversity. The lima bean seed from a specific region might have different characteristics from others. This study was aimed to characterize the physicochemical and protein of the dry seed of lima bean from Indonesia based on its solubility electrophoretic pattern and amino acid profile. The result showed that carbohydrate (68.891.55) was the major component with starch (41.961.10) as the predominant. The amylopectin was higher than amylose. Dietary fibre (27.870.37) was significant and dominated by insoluble one (25.470.32). The fat content (1.150.04) was low and ash (3.670.47) comprised of magnesium phosphorus potassium calcium and iron with the content of 184 75 38 11 and 10 mg/100 g respectively. Total phenolic compounds of this seed were 1.290.02 mg/g phytic acid 11.570.03 mg/g saponins 16.840.42 mg/g and trypsin inhibitors 36.070.11 TIU/g. HCN was found significantly at 30.990.29 ppm. Oligosaccharides were 5.930.29 that comprised of raffinose 1.220.08 and stachyose 4.610.21. Protein content was moderate (15.930.55) that comprised of albumin 18.470.62 globulin 56.202.00 prolamin 3.140.20 and glutelin 22.691.60. The molecular weight of this protein was 10-141 kDa with 12 polypeptides. Globulin had 12 polypeptides of 10 to 124 kDa. Albumin had 18 to 116 kDa molecular weights while glutelin had six polypeptides of 13 to 109 kDa. Prolamin did not have visible polypeptides. Lysine leucine valine and phenylalanine were the primary essential amino acids with high lysine but low methionine and cysteine. In conclusion lima bean seed is a source of carbohydrates and minerals with a moderate protein content dominated by the globulin. The polypeptide profile of lima bean seed is varied depending on the protein fractions. The occurrence of antinutrition might hinder its utilization as a protein source. HCN as a toxicant should be removed to obtain a safe seed for consumption.

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Item Type:	Article
AGROVOC Term:	Lima beans
AGROVOC Term:	Phaseolus lunatus
AGROVOC Term:	Proteins
AGROVOC Term:	Legumes
AGROVOC Term:	Food composition
AGROVOC Term:	Proximate composition
AGROVOC Term:	Chemical analysis (methods)
AGROVOC Term:	Proximate analysis
AGROVOC Term:	Physicochemical properties
AGROVOC Term:	Food nutrient content
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10777

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