In vitro propagation of Bambusa balcooa by plant tissue culture technique

Anbuselvi S., . and Priyanka P. S., . and Monitha B., . and Saroja Preethy R., . In vitro propagation of Bambusa balcooa by plant tissue culture technique. pp. 124-127. ISSN 2550-2166

Bambusa balcooa is a common plant grown in Bihar Orissa Jharkhand and Uttarakhand states of India having good cultural importance. The present experimentation on nodal explants of B. balcooa on MS media supplement under particular culture conditions has shown shunted growth in 30 days. Shoot tip explants of B. balcooa seedlings produced multiple shoots on MS medium supplemented with different plant growth regulators (PGRs) individually and in combination. Shoot tip explants of B. balcooa requires 30 days to initiate shoots. Among the three cytokinins tested BAP was selected as the most suitable hormone to induce shoot multiplication. The highest shoot multiplication is found by incorporation with BAP (1.5 g/L and 2 g/L). The shoot multiplication rates are good with shoot length 2.50.3 cm and 3.90.4 cm with the highest and maximum shoot generation. The shoot multiplication rate is moderate with a shoot length of 2.000.5 cm. Multiplication potentiality was observed in the cluster having more than 2“3 shoots. The best period for recycling multiplying shoots is 2“3 weeks in the old culture. Delaying of the sub-culturing period resulted in gradual browning of the shoots. The sub-culturing period was recorded as the most crucial factor for obtaining the optimal and desired level of regeneration of shoots. The well-grown propagules in the present experimentation were shown successful regeneration. Hence the experiment concluded with the best growth pattern observed in media containing auxins like BAP and cytokinins like kinetin.

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Abstract

Bambusa balcooa is a common plant grown in Bihar Orissa Jharkhand and Uttarakhand states of India having good cultural importance. The present experimentation on nodal explants of B. balcooa on MS media supplement under particular culture conditions has shown shunted growth in 30 days. Shoot tip explants of B. balcooa seedlings produced multiple shoots on MS medium supplemented with different plant growth regulators (PGRs) individually and in combination. Shoot tip explants of B. balcooa requires 30 days to initiate shoots. Among the three cytokinins tested BAP was selected as the most suitable hormone to induce shoot multiplication. The highest shoot multiplication is found by incorporation with BAP (1.5 g/L and 2 g/L). The shoot multiplication rates are good with shoot length 2.50.3 cm and 3.90.4 cm with the highest and maximum shoot generation. The shoot multiplication rate is moderate with a shoot length of 2.000.5 cm. Multiplication potentiality was observed in the cluster having more than 2“3 shoots. The best period for recycling multiplying shoots is 2“3 weeks in the old culture. Delaying of the sub-culturing period resulted in gradual browning of the shoots. The sub-culturing period was recorded as the most crucial factor for obtaining the optimal and desired level of regeneration of shoots. The well-grown propagules in the present experimentation were shown successful regeneration. Hence the experiment concluded with the best growth pattern observed in media containing auxins like BAP and cytokinins like kinetin.

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Item Type:	Article
AGROVOC Term:	Bambusa
AGROVOC Term:	Bamboos
AGROVOC Term:	Culture techniques
AGROVOC Term:	Plant tissues
AGROVOC Term:	In vitro culture
AGROVOC Term:	Plant propagation
AGROVOC Term:	Cytokinins
AGROVOC Term:	Auxins
AGROVOC Term:	Soil conservation
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10808

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