Extraction optimisation of total polyphenols from Bletilla striata by high-shear dispersing emulsification technology and their anti-melanogenesis activity


Citation

Du T. Y., . and Yu C. H., . and Kwon T. H., . and Ke Z. C., . and Cheng Z. Y., . and Zhang G., . and Wang W. D., . Extraction optimisation of total polyphenols from Bletilla striata by high-shear dispersing emulsification technology and their anti-melanogenesis activity. pp. 752-763. ISSN 22317546

Abstract

Bletilla striata is a good source of plant polyphenols which have regulatory functions on melanogenesis. In the present work to maximise the yields of total polyphenols extracted from B. striata the effects of Tween-60 concentration shear speed shear time ethanol concentration temperature and material-to-solvent ratio on extraction of polyphenols were assayed using the high-shear dispersing emulsification technology (HSDE). The obtained data were analysed using the response surface methodology which showed that the optimal extraction conditions were as follows: Tween-60 concentration of 2.00 ethanol concentration of 69 shear speed of 17 200 rpm and extraction temperature of 55C. Under these conditions the extraction of the total polyphenols from B. striata was 13.79 0.34 mg/g. Furthermore the anti-melanogenesis activity of the total polyphenols extracted from B. striata was evaluated using -MSH-induced B16F10 cells. Results showed that the total polyphenols from B. striata could significantly inhibit melanin production. Overall these results presented the optimal conditions for the extraction of total polyphenols from B. striata and also revealed the potential anti-melanogenesis values of B. striata polyphenols.


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Abstract

Bletilla striata is a good source of plant polyphenols which have regulatory functions on melanogenesis. In the present work to maximise the yields of total polyphenols extracted from B. striata the effects of Tween-60 concentration shear speed shear time ethanol concentration temperature and material-to-solvent ratio on extraction of polyphenols were assayed using the high-shear dispersing emulsification technology (HSDE). The obtained data were analysed using the response surface methodology which showed that the optimal extraction conditions were as follows: Tween-60 concentration of 2.00 ethanol concentration of 69 shear speed of 17 200 rpm and extraction temperature of 55C. Under these conditions the extraction of the total polyphenols from B. striata was 13.79 0.34 mg/g. Furthermore the anti-melanogenesis activity of the total polyphenols extracted from B. striata was evaluated using -MSH-induced B16F10 cells. Results showed that the total polyphenols from B. striata could significantly inhibit melanin production. Overall these results presented the optimal conditions for the extraction of total polyphenols from B. striata and also revealed the potential anti-melanogenesis values of B. striata polyphenols.

Additional Metadata

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Item Type: Article
AGROVOC Term: Orchidaceae
AGROVOC Term: Herbaceous plants
AGROVOC Term: Polyphenols
AGROVOC Term: Emulsification
AGROVOC Term: Extraction
AGROVOC Term: Secondary metabolites
AGROVOC Term: Viability
AGROVOC Term: Tyrosinases
AGROVOC Term: Melanins
AGROVOC Term: Phenolic compounds
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:55
URI: http://webagris.upm.edu.my/id/eprint/10817

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