Multiplex PCR assay for species identification of meat and dairy products from buffalo (Bubalus bubalis) cattle (Bos indicus and Bos taurus) goat (Capra hircus) and sheep (Ovis aries)

Suwannarat S., . and Sangthong D., . and Samipak S., . and Sangthong P., . Multiplex PCR assay for species identification of meat and dairy products from buffalo (Bubalus bubalis) cattle (Bos indicus and Bos taurus) goat (Capra hircus) and sheep (Ovis aries). pp. 716-725. ISSN 22317546

Cases of fraudulent meat and dairy products have increased worldwide especially in developing countries. To determine the misrepresented animal species appropriate tools in routine monitoring should be available for food inspections. In the present work a multiplex polymerase chain reaction assay for species identification of products from ruminants including buffalo cattle goat and sheep was developed. The primer set KUMUT_cFarmSp1 was composed of five species-specific primers and a pair of positive-control primers. The primer set amplified 106- 163- 232- and 308-bp specific fragments from the cytochrome b (cyt b) gene of buffalo cattle goat and sheep respectively and 370-bp positive-control fragment from 16S ribosomal RNA (16S rRNA). The detection limit of this PCR assay is 0.1 ng of DNA template. The developed primer set exhibited strong specificity sensitivity robustness and simplicity for food verification thus indicating its usefulness for species verification in food quality control and law enforcement.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/28%20(04)%202021/DONE%2...

Abstract

Cases of fraudulent meat and dairy products have increased worldwide especially in developing countries. To determine the misrepresented animal species appropriate tools in routine monitoring should be available for food inspections. In the present work a multiplex polymerase chain reaction assay for species identification of products from ruminants including buffalo cattle goat and sheep was developed. The primer set KUMUT_cFarmSp1 was composed of five species-specific primers and a pair of positive-control primers. The primer set amplified 106- 163- 232- and 308-bp specific fragments from the cytochrome b (cyt b) gene of buffalo cattle goat and sheep respectively and 370-bp positive-control fragment from 16S ribosomal RNA (16S rRNA). The detection limit of this PCR assay is 0.1 ng of DNA template. The developed primer set exhibited strong specificity sensitivity robustness and simplicity for food verification thus indicating its usefulness for species verification in food quality control and law enforcement.

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Item Type:	Article
AGROVOC Term:	Buffaloes (bubalus)
AGROVOC Term:	Bos indicus
AGROVOC Term:	Bos taurus
AGROVOC Term:	Capra hircus
AGROVOC Term:	Ovis aries
AGROVOC Term:	Sampling
AGROVOC Term:	Laboratory experimentation
AGROVOC Term:	Food quality
AGROVOC Term:	Consumer protection
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10835

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