Citation
Koh W Y, . and Chan L K, . and Tai W Y, . and Su T. S ., . (2000) Comparative study of micropropagation techniques on Typhonium flagelliforme. [Proceedings Paper]
Abstract
A micropropagation technique by means of aeration was tested on Typhonium flagelliforme and compared with other conventional micropropagation techniques such as the solid culture technique and shake culture technique. Aeration technique stimulates multiple bud formation more rapidly than solid culture technique and the shake culture technique. Shoots of T. flagelliforme produced 2.5 buds per shoot when cultured by aeration technique in 3 weeks compared with 1.6 buds per shoot by solid culture technique and only 1.2 buds per shoot by shake culture technique. Shoots of T. flagelliforme produced 4.0 buds which is significantly more than multiple buds formed from tuber tissues. Shoots of T. flagelliforme produced the most multiple buds when cultured in modified liquid MS medium X with 40g/1 sucrose after 4 weeks in aeration tubes. In this liquid medium light intensity of 800 lux is adequate for the highest multiple bud formation. This study also showed that 5 explants in one aeration tube are most suitable for multiple bud formation. The aeration rate of 25.3 ml/min is sufficient to produce high number of multiple buds.
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Abstract
A micropropagation technique by means of aeration was tested on Typhonium flagelliforme and compared with other conventional micropropagation techniques such as the solid culture technique and shake culture technique. Aeration technique stimulates multiple bud formation more rapidly than solid culture technique and the shake culture technique. Shoots of T. flagelliforme produced 2.5 buds per shoot when cultured by aeration technique in 3 weeks compared with 1.6 buds per shoot by solid culture technique and only 1.2 buds per shoot by shake culture technique. Shoots of T. flagelliforme produced 4.0 buds which is significantly more than multiple buds formed from tuber tissues. Shoots of T. flagelliforme produced the most multiple buds when cultured in modified liquid MS medium X with 40g/1 sucrose after 4 weeks in aeration tubes. In this liquid medium light intensity of 800 lux is adequate for the highest multiple bud formation. This study also showed that 5 explants in one aeration tube are most suitable for multiple bud formation. The aeration rate of 25.3 ml/min is sufficient to produce high number of multiple buds.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 A1S471 2000 Call Number. |
| AGROVOC Term: | MICROPROPAGATION |
| AGROVOC Term: | AERATION |
| AGROVOC Term: | BUDS |
| AGROVOC Term: | CYCLONES |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:13 |
| URI: | http://webagris.upm.edu.my/id/eprint/11292 |
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