Preliminary screening of antiproliferative activity of selected extracts of Centella asiatica

Azimahtol Hawariah L. P., . and Nur Kartinee K., . and Azizol A. K., . (2000) Preliminary screening of antiproliferative activity of selected extracts of Centella asiatica. [Proceedings Paper]

Centella asiatica is a tropical plant found in Madagascar and Asia particularly Indonesia India and Malaysia. Its benefits for health have long been known in its native areas. Our elders use C. asiatica to cure a number of skin problems and heal small wounds. The present study examines the anti-proliferative effect of the crude extracts of C. asiatica as well as its partially purified fractions CF from silica gel column chromatographic procedures in vitro. The method used to verify the anti-proliferative activity was methylene blue staining assay by counting the number of living cells at the time of assay termination. A total of 11 accessions of C. asiatica methonolic crude extracts MC were subjected to a preliminary anti-proliferative bioassay screening against breast cancer MCF-7 prostate cancer DC145 ovarian cancer CAOV-3 oral epidemoid KB and rybdosarcoma RD cell lines and compared with normal cell lines MDBK and VERO. Only CA-03 w Ip had shown activity with IC50 values of 168 178.5 89.11 and 198 ug/ml on cell lines of KB CaOV-3 MCF-7 and DUl45 respectively. No activity was detected for the rest of the extracts even at concentration as high as 200 ug/ml. The ethanolic crude extract EC of CA-03 wp when tested on CaOV-3 MCF-7 and DU 145 gave IC50 values at 99 125.8 180 ug/ml and greater than 200 ug/ml respectively. The chloroform fraction CF showed good anti-proliferative activity on CaOV-3 with IC50 of 27.5 ug/ml. The other IC50 for CF were 36.3 45 and 110 ug/ml on DU145 MCF-7and RD respectively. All the C. asiatica extracts were non-toxic to the normal cell lines.

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Abstract

Centella asiatica is a tropical plant found in Madagascar and Asia particularly Indonesia India and Malaysia. Its benefits for health have long been known in its native areas. Our elders use C. asiatica to cure a number of skin problems and heal small wounds. The present study examines the anti-proliferative effect of the crude extracts of C. asiatica as well as its partially purified fractions CF from silica gel column chromatographic procedures in vitro. The method used to verify the anti-proliferative activity was methylene blue staining assay by counting the number of living cells at the time of assay termination. A total of 11 accessions of C. asiatica methonolic crude extracts MC were subjected to a preliminary anti-proliferative bioassay screening against breast cancer MCF-7 prostate cancer DC145 ovarian cancer CAOV-3 oral epidemoid KB and rybdosarcoma RD cell lines and compared with normal cell lines MDBK and VERO. Only CA-03 w Ip had shown activity with IC50 values of 168 178.5 89.11 and 198 ug/ml on cell lines of KB CaOV-3 MCF-7 and DUl45 respectively. No activity was detected for the rest of the extracts even at concentration as high as 200 ug/ml. The ethanolic crude extract EC of CA-03 wp when tested on CaOV-3 MCF-7 and DU 145 gave IC50 values at 99 125.8 180 ug/ml and greater than 200 ug/ml respectively. The chloroform fraction CF showed good anti-proliferative activity on CaOV-3 with IC50 of 27.5 ug/ml. The other IC50 for CF were 36.3 45 and 110 ug/ml on DU145 MCF-7and RD respectively. All the C. asiatica extracts were non-toxic to the normal cell lines.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 A1S471 2000 Call Number.
AGROVOC Term:	NEOPLASMS
AGROVOC Term:	TISSUE PROLIFERATION
AGROVOC Term:	UMBELLIFLORAE
AGROVOC Term:	IN VITRO
AGROVOC Term:	BIOCHEMISTRY
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11318

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